CTLA_4Ig基因表达对鼠心脏移植免疫抑制作用的研究

ADENOVIRUS-MEDIATED TRANSFER OF CTLA_4Ig GENE RESULTS IN PROLONGED SURVIVAL OF HEART ALOGRAFT

大鼠 DA和 L ewis分别作为移植的供受体。心脏移植前 1周 ,用腺病毒作载体将 CTL A4Ig基因导入 L ewis鼠体内 ,研究该基因在鼠体内的表达以及对同种心脏移植后的免疫抑制作用。以编码 β-半乳糖苷酶有复制缺陷的腺病毒重组体 ( Adex/L ac E)为对照组。RT-PCR法检测基因的表达 ,并用流式细胞仪测定 CTL A4Ig蛋白质浓度变化。用移植物生存时间判断免疫抑制效果。结果显示 :导入鼠体内的 CTL A4Ig基因能够在鼠的肝脏中表达 ,并使其表达产物 CTLA4Ig在血中浓度明显高于对照组 ( P<0 .0 5 )。而且移植心脏的生存时间显著长于对照组 ( P<0 .0 5 )。实验表明 CTLA4Ig基因能在鼠体内良好表达并对同种移植的排斥反应有明显的抑制作用。

The study of CTLA 4Ig's expression and its products immunoinhibition after the CTLA 4Ig gene cloned in adenovirus was introduced into the recipient rat during heart allograft.The replication-defective recombinant adenovirus encoding the Escherichia coli Lacz gene,cable of producing the enzyme β-galactosidase was used as control.RT-PCR was employed to detect the expression of CTLA 4Ig gene in the recipient rat and,flow cytometer was used to monitor the blood concentration of CTLA 4Ig product.The experiments showed that the transfected CTLA 4Ig gene could express highly in the recipient rat liver and its products were able to remarkably prolong survival of heart allograft(P<0.05).Therefore CTLA 4Ig can be considered to have strong inhibitory effect on the immune rejection of allograft organs. [