不同种系动物诱导实验性变态反应性脑脊髓炎模型的研究

STUDY OF VARIOUS KINDS OF ANIMALS INDUCING EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

目的 :探索国内不同种系动物诱发实验性变态反应性脑脊髓炎 ( EAE)的可能性 ,寻找诱导 EAE的合适实验动物 ,以推进国内临床神经免疫学的研究。方法 :用含异种动物脊髓的完全弗氏佐剂乳化物作为抗原 ,进行动物背部皮下多点注射 ,观察和比较动物的神经症状和病理变化。结果 :( 1)抗原诱导 EAE,如果发病 ,一般都在注射抗原 2周后开始出现症状 ,在注射后 19天左右症状最明显 ,且脑组织出现明显的炎性病理变化。 ( 2 ) SD大鼠的发病率为 13 %,Wistar大鼠的发病率为 3 0 %,昆明种 ( KM)小鼠的发病率为 0。 ( 3 )未发病的 Wistar大鼠再次注射抗原 ,观察 2个月 ,仍未发病。结论 :利用 Wistar大鼠制备 EAE动物模型在国内较适宜 ,而EAE发病的个体差异可能主要受遗传基因的控制。

Objective: Experimental allergic encephalomyelitis (EAE), a T-cell mediated autoimmune disease, is widely considered as an animal model of multiple sclerosis. Various kinds of animals were used to induce EAE in order to search a domestic rat with high EAE incidence. Methods: Various kinds of animals were injected in 5 to 6 locations of the back of the body 0.5 ml with the guinea pig spinal cord in Freund's complete adjuvant and observed the clinical symptoms and histological alterations. Results:(1) The clinical symptoms of EAE of SD and Wistar rat occurred in two weeks after the rats were injected with antigens, the peak of the clinical symptoms and histological alterations on the 19th days.(2) The SD rat incidence of EAE was 13%, Wistar rat incidence of the disease was 30% and Kunming mouse incidence of the disease was 0%.(3) The Wistar rats without coming on EAE were secondly injected the antigen and observed for two months, EAE having still not occurrence.Conclusion: Wistar rat may be a fitter animal inducing EAE model in domestic usual experimental animals. The rat individual differences of EAE incidence may be mainly controlled by hereditary genes. [