CXCR4-shRNA对人胃癌细胞SGC7901裸鼠腹腔转移瘤生长转移影响的实验研究

CXCR4-shRNA inhibits growth and metastasis of human gastric carcinoma SGC7901 cell in nude mice

目的探讨CXCR4-shRNA腺病毒载体对人胃癌细胞裸鼠腹腔转移瘤的抑制作用。方法构建裸鼠腹腔转移瘤模型,分为SGC7901组、空病毒组、CXCR4-shRNA组,比较3组裸鼠肿瘤体积、重量,转移灶肿瘤数,裸鼠生存时间、生存延长率、抑瘤率。Western blot检测肿瘤组织VEGF-C、MPP-2蛋白的表达情况。结果接种8～15d后裸鼠均有肿瘤长出,术区可触及大小2～3cm的结节,质硬且活动不良。shRNA组肿瘤体积平均(0.78±0.14)mm3、重量平均(1.20±0.16)g、数目平均(5.75±1.39)个,与SGC7901组[(5.61±0.44)mm3、(3.93±0.19)g、(28.86±1.83)个]及空病毒组[(4.65±0.42)mm3、(3.82±0.14)g、(27.75±1.39)个]比较差异有统计学意义(P﹤0.01);SGC7901组及空病毒组比较,差异无统计学意义(P﹥0.05)。SGC7901组生存时间平均(25.50±1.28)d,空病毒组(29.00±1.60)d,CXCR4-shRNA组(40.62±2.06)d;CXCR4-shRNA组生存延长率及抑瘤率分别为59.3%及69.5%,空病毒组分别为13.7%及2.8%。Westernblot检测结果显示,CXCR4-shRNA组VEGF-C、MMP-2蛋白表达水平明显弱于SGC7901组及空病毒组。结论 CXCR4-shRNA腺病毒载体可抑制人胃癌细胞裸鼠腹腔转移瘤的生长及转移。

Objective To investigate the inhibitory effect of the CXCR4-shRNA adenovirus vector on the growth and metastasis of transplanted tumors of human gastric cancer cells in nude mice.Methods Three groups of nude mice tumor model with abdominal cavity metastasis were established：the SGC7901 group,the vector virus group,the CXCR4-shRNA group.The diameter,weight of the tumor and the number of metastatic tumor in the three groups of nude mice tumor were compared.Their survival time,survival rate,life extension rate were compared.Western blot was used to detect the VEGF-C and MMP-2 protein expression levels.Results Compared with the SGC7901 group and the vector virus group,the ascites production time in CXCR4-shRNA group nude mice was significantly delayed and less,the diameter and weight of tumor were decreased,as well as the survival time was extended.In addition,the mortality was reduced in the nude mice.The protein expression levels of the VEGF-C,MMP-2 were inhibited.Conclusion CXCR4-shRNA adenovirus vector can inhibit growth and metastasis of human gastric carcinoma SGC7901 cell in nude mice.