肝外胆管癌组织中Ki-67抗原的表达及临床意义

ASSESSMENT OF THE EXPRESSION OF Ki-67 ANTIGEN IN CARCINOMA OF THE EXTRAHEPATIC BILE DUCT

目的 :探讨 Ki-67抗原在肝外胆管癌组织中的表达及其临床应用价值。方法 :采用免疫组化技术 ( S-P法 )检测 3 5例肝外胆管癌石蜡包埋标本中 Ki-67的表达 ,与 12例慢性胆管炎标本作对照。结果 :肝外胆管癌组织中 Ki-67标记指数 ( Ki-67LI)为 3 4 .6± 19.5 ,显著高于胆管炎组织的 16.3± 12 .8( P<0 .0 1)。 Ki-67LI与肝外胆管癌病理类型无关 ,但在浸润深度 T1 、T3期 ,临床分期 、 期与 、 期及淋巴结转移之间均表现出显著差异。 Ki-67LI与病人术后生存期呈显著负相关 ,Ki-67LI越高 ,病人术后生存期越短 ,预后越差。结论 :Ki-67检测对临床评估肝外胆管癌病情 ,判断病人预后及选择辅助治疗方法有一定的指导意义。

Objective:To investigate the expression of Ki-67 antigen in carcinoma of the extrahepatic bile duct(EHBD)and its clinical implication.Methods:Formalin fixed,paraffin embedded specimens from 35 patients with EHBD carcinoma were immunostained with Ki-67 antibody by S-P immunohistochemical technique,compared with 12 patients with cholangitis.Results:Ki-67 labeling index (Ki-67 LI)was 34.6±19.5 in EHBD carcinoma and 16.3±12.8 in cholangitis.The differences between them were significant(P<0.01).The Ki-67 LI was not related to histologic type.Significant differences were observed in relation to invasive depth,clinical stage,lymph node metastasis.There were significant differences in the survival time between the group,with Ki-67 LI being ≥34.6 and the group,being ≤34.6.The higher the Ki-67 LI,the shorter the patients survive,indicating the poor prognosis of the patients.Conclusions:The results of this study indicated that detection of Ki-67 antigen might be useful in assessing the development,judging prognosis,selecting clinical therapy of patients with EHBD carcinoma. [