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泛耐药鲍曼不动杆菌耐药机制和传播机制研究 被引量：13

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摘要 目的:了解我院泛耐药鲍曼不动杆菌的耐药机制和传播机制。方法:利用PCR、RFLP分型和测序技术对临床分离的77株泛耐药鲍曼不动杆菌进行整合子I、ISCR1和常见碳青霉烯酶基因研究,并利用ERIC-PCR研究其传播机制。结果:77株菌中58株(75%)整合酶I阳性,50株(65%)整合子I阳性;48株(62%)ISCR1阳性,45株(58%)ISCR1携带qnrA1和ampR耐药基因盒;8株检出复杂性整合子I;1株检出NDM-1基因,62株(80%)检出OXA-23-like基因,4株(5%)检出OXA-58-like,OXA-51-like基因100%携带,未检出KPC和OXA-24-like基因。ERIC-PCR聚类分析60%的相似水平上被聚为16个类群。结论:整合子I、ISCR1和OXA类酶在鲍曼不动杆菌介导多重耐药方面有重要作用,ERIC-PCR是进行泛耐药鲍曼不动杆菌同源性分析的有效方法。
作者 郑芬 程灿灿 芮勇宇
机构地区 南方医科大学南方医院检验科
出处 《实用医学杂志》 CAS 北大核心 2013年第9期1510-1512,共3页 The Journal of Practical Medicine
基金 广东省自然科学基金项目(编号:S2012010009153) 南方医院新业务新技术课题(编号:[2012]4号201116)
关键词 泛耐药鲍曼不动杆菌 整合子 ISCR1 碳青霉烯酶 ERIC-PCR
分类号 R446.5 [医药卫生—诊断学]
  • 相关文献

参考文献11

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二级参考文献12

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共引文献7

同被引文献114

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引证文献13

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实用医学杂志
2013年 第9期

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