Ras/NF-IL-6信号转导途径介导人骨髓瘤细胞KM-3中的IL-6生物学效应

The biological function of IL-6 on a human multiple myeloma cell-KM-3 is mediated by Ras/NF-IL-6 signal transduction pathway

目的 研究人骨髓瘤细胞KM 3中的IL 6信号转导途径与生物学效应之间的关系。方法 分别采用MTT、凝胶阻滞电泳 (electrophoreticmobilityshiftassay ,EMSA)、免疫沉淀方法检测IL 6对KM 3细胞生长状态的影响、参与IL 6信号传递功能的转录因子以及蛋白激酶在KM 3细胞中的诱导激活状态。结果 IL 6可明显促进KM 3细胞增殖 ,并可引发Ras/NF IL 6信号转导途径活化 ,但Jak/STAT信号途径没有激活。结论 Ras/NF IL 6信号转导途径介导了IL 6对KM

Objective To investigate the relationship between the IL 6 signal transduction pathways and its biological function on a human multiple myeloma cell line KM 3. Methods MTT, EMSA and immunoprecipitation were used respectively to detect the effect of IL 6 on the growth of KM 3 cell, the activation of transcription factors and protein kinase in the two IL 6 signal transduction pathways by IL 6 in the same cells. Results IL 6 can promote the proliferation of KM 3 cells and activate one of the IL 6 signal transduction pathway Ras/NF IL 6; but the Jak/STAT signal transduction pathway was not activated at the same conditions. Conclusion The biological function of IL 6 on the KM 3 cells is mediated by Ras/NF IL 6 signal transduction pathway.