外周血淋巴细胞携带风疹抗原与中枢神经感染风疹病毒的关系

Antigen carry by peripheral lymphocytes and rubella virus infection in central nervous system

目的 探讨外周血淋巴细胞携带风疹病毒抗原与风疹病毒感染中枢神经系统的关系。方法 BALB/c小鼠分别给予临床常用的可影响机体免疫功能的药物 ,再经腹腔感染风疹病毒 ,并在感染后的 1、3、7、14d观察外周血淋巴细胞携带病毒抗原的情况 ,分析其与中枢神经系统病毒感染的关系。结果 地塞米松药物组在不同时间抗原的平均携带率分别为 3 1%、4 1%、9 6 %、2 4% ,环磷酰胺药物组分别为 14 2 %、12 7%、9 9%、3 1% ,未用药物干预的感染组分别为 4 6 3 %、10 2 5 %、6 88%、1 75 %。方差分析显示 ,3组动物在感染风疹病毒后的第 2 4小时外周血淋巴细胞携带抗原存在明显差异 ,F =0 0 317,P <0 0 5。组间两两比较结果显示 :环磷酰胺药物组动物外周血淋巴细胞抗原的携带明显高于其它实验动物组 ,地塞米松药物组和药物未干预组动物之间无明显差异。确切概率法分析表明 ,动物外周血淋巴细胞风疹病毒的持续性携带与中枢神经系统风疹病毒感染的关系极为密切 ,P <0 0 0 1。结论 环磷酰胺可能影响风疹病毒感染后外周血淋巴细胞对病毒的携带率。在感染初期 。

Objective To investigate the correlation between rubella virus antigen carry by peripheral lymphocytes and rubella virus infection in central nervous system(CNS). Methods BALB/c mice were used as a model and treated with ordinary medicines affecting immunological functions. And then, the mice were infected via abdominal cavity and the antigen carry situations in peripheral lymphocytes were examined at 1,3,7 and 14 days of the infection. Rubella viruses in CNS were detected by immunohistochemical methods. Results The mean antigen carry rates at different times are 3.1%, 4.1%, 9.6% and 2.4%, respectively in the dexamethasone group, 14.2% , 12.7%, 9.9% and 3.1%, respectively in the cytoxan group. In the control group without any intervention, the rates are 4.63%, 10.25%, 6.88% and 1.75%, respectively. The antigen carry rates in peripheral lymphocytes among the three groups at 24 hours post infection are significantly different ( F =0.0317, P <0.05). Comparisions between groups showed that antigen carry rates in cytoxan group are much higher than those in other groups, but there was no difference between dexamethasone and control groups. The animals with persistent antigen carry were much easier to get cerebral infection than those with short term carry ( P <0.001). Conclusions Cytoxan can affect virus carry rates in peripheral lymphocytes. At the early phase of infection, persistent carry of rubella virus in peripheral lymphocytes increases the opportunity of getting central nervous system infection.