银杏内酯B对小鼠胚胎着床作用的影响

Effect of ginkgolide B on embryo implantation of mice

目的:了解银杏内酯B(GB)对小鼠胚胎着床的影响。方法:未经产雌性昆明小鼠,于妊娠第2天一侧子宫角宫腔内注射不同剂量的GB(2、4、6、8、10mg/kg)作为实验侧,同一小鼠另一侧子宫角宫腔内注射相应体积的生理盐水作为对照侧。于妊娠第8天脱颈椎法处死小鼠,立即剖腹观察并记录胚胎数目。结果:GB2mg/kg组实验侧宫腔胚胎数与对照侧无差异(P>0.05);4mg/kg、6mg/kg组实验侧宫腔胚胎数目少于对照侧(P均<0.05),8mg/kg、10mg/kg组实验侧宫腔胚胎数目少于对照侧(P均<0.001)。5个剂量组的实验侧,GB2mg/kg组胚胎数目多于其他4个剂量组(分别为P<0.05和P<0.001),4mg/kg组与6mg/kg组之间无差异,8mg/kg组与10mg/kg组之间无差异,但4mg/kg、6mg/kg剂量组分别与8mg/kg、10mg/kg剂量组比较差异有统计学意义(P均<0.001),前者胚胎数目显著多于后者。结论:GB可以干扰小鼠胚胎着床,且效果与剂量相关。

Objective： To investigate the influence of ginkgolide B upon mice embryo implantation. Methods：Kunming female mice without producing, on the 2nd day of pregnancy, were given ginkgolide B in different doses in one side of the uterine horn （ 2mg/kg, 4 mg/kg, 6 mg/kg, 8 mg/kg, 10 mg/kg） as the experimental side, while equal amount of physiological saline was injected into the uterine horn of the other side as the control side. On the 8th day of pregnancy, the mice were executed for counting the number of embryos. Results ： The number of embryos of the test side was a little bit less than that of the control side in the 2mg/kg group （ P 〉 0.05 ）, but less than those of the control sides in the 4 mg/kg and the 6 mg/kg groups （ P 〈 0.05 ）, and very significantly less than those of the control sides in the 8 mg/kg and the 10 mg/kg groups （ P 〈 0. 001 ）. In 5 different doses groups of the experimental sides, the number of embryos of the 2mg/kg group was much more than those of the other 4 groups （P 〈0. 05, P 〈0. 001, respectively）. And compared with the 8 mg/kg and the 10 mg/kg groups, the number of embryos of the 4 mg/kg and the 6 mg/kg groups were much move （ P all 〈0. 001 ）, though there were no differences be-tween the 4 mg/kg and the 6 mg/kg, the 8 mg/kg and the 10 mg/kg group. Conclusion： Ginkgolide B can inhibit the embryo implantation of the mice, and the effect is correlated with the doses.