卵巢癌患者血清CA125、HE4和OPN的表达及意义

Expression of Serum CA125, HE4 and OPN in Patients with Ovarian Cancer and Its Signifi- cance

【目的】探讨卵巢癌患者血清糖类抗原125（CAl25）、骨桥蛋白（OPN）、人附睾分泌蛋白4（HE4）的表达及意义。【方法】选择2010年6月至2012年7月本院妇科收治的卵巢癌患者35例（A组）、卵巢良性肿瘤患者73例（B组）及40例同期体检的健康妇女（C组）为研究对象，应用电化学发光法检测三组患者手术前后血清CA125水平，ELISA法检测患者手术前后血清HE4、OPN水平，比较三种肿瘤标志物单项及联合检测在卵巢癌诊断中的特异性及敏感性。【结果】A组术前血清CAl25、HE4和OPN水平分别为（98．64±220．91）U／mL（412．3±278．62）μmol／mL和（94．6±61．06）ng／mL，与B组、C组比较差异均有统计学意义（P〈0．05）；A组手术前血清CAl25、HE4和OPN水平与术后1个月比较，差异均有统计学意义（P〈0．05）。联合检测诊断卵巢癌的特异性（78．1％）稍低于血清cAl25、HE4和OPN单项指标检测（80．8％、100％、87．6％），但比较差异无统计学意义（P〉0．05），而血清联合检测诊断卵巢癌的敏感性（94．3％）显著高于血清CAl25、HE4和OPN单项指标检测（77．1％、71．4％、37．1％），其差异有统计学意义（P〈0．05）。【结论】联合检测卵巢癌患者血清CAl25、HE4和OPN水平可作为诊断和评估卵巢癌预后的参考指标。

[Objective] To explore the expression of serum carbohydrate antigen 125 （CA125）, human epi- didymis secreting protein 4（HE4） and osteopontin（OPN） in patients with ovarian cancer and its significance. [Methods]Totally 35 patients with ovarian cancer（group）, 73 patients with ovarian benign tumor（group B） and 40 healthy women（group C） in gynecology department of our hospital from June 2010 to July 2012 were enrolled in the study. Serum CA125 in patients of three groups was detected by electrochemiluminescence method before and after operation. Serum levels of HE4 and OPN in patients before and after treatment were measured by enzyme-linked immunosorbent assay（ELISA）. The sensitivity and specificity of single and com- bined detection of these markers in the diagnosis of ovarian cancer were compared. [Results] The levels of ser- um CA125, HE4 and OPN in group A were （98.64~220.91） U/mL, （412.3_＋278.62） pmol/mL and （94.6 !61.06） ng/ml respectively, and there were significant differences（ P dO. 05）. There was significant differ- ence in serum CA125, HE4 and OPN in group A between before operation and one month after operation（ P d0.05）. The specificity of the combined detection in the diagnosis of ovarian cancer（78. 1%） was slightly lower than that of single detection of CA125, HE4 and OPN（80.8%, 100% and 87.6%, respectively）, but there was no significant difference（ P 〈0.05）. The sensitivity of the combined detection in the diagnosis of o- varian cancer（94.3%） was significantly higher than that in single detection of serum CA125, HE4 and OPN （77.1%, 71.4% and 37.1%, respectively）, and there Was significant difference（ P 〈0.05）. [Conclusion] The joint detection of serum CA125, HE4 and OPN can be used as the reference index of the diagnosis and prognosis in patients with ovarian cancer.