肝细胞癌组织中miR-218的表达及其临床意义

Expression of miR-218 in Hepatocellular Carcinoma and Its Clinical Significance

【目的】研究miR-218在肝细胞癌（Hepatocellularcarcinoma，HCC）组织中的表达并探讨其临床意义。【方法】收集HCC手术组织标本54例，30例癌旁正常组织作为对照，采用实时荧光定量PCR（qRT-PCR）检测HCC组织及对照组织中miR-218的表达情况，分析miR_218表达水平与HCC临床病理参数之间的关系。【结果】与癌旁对照组织相比，miR-218在HCC组织表达普遍下调，差异具有统计学意义（P〈0．01），癌旁对照组织中miR-218平均表达量约为HCC的2．90倍；miR-218下调表达程度与HCC恶性程度、肿瘤分化程度、肝内和肝外转移及门静脉癌栓有关，miR_218的表达水平与HCC患者年龄、性别、饮酒史、HBsAg、肿瘤大小、是否合并肝硬化等无关。【结论]miR-218与HCC的发生、临床进展密切相关，其可能成为HCC新的潜在的治疗靶点及诊断和预后的分子标志物。

[Objective] To investigate the expression of miR-218 in hepatocellular carcinoma（HCC）and its clinical significance. [Methods] Totally 54 cases of HCC biopsies were collected. And 30 normal peri-carcino- ma tissues were selected as the control. Real-time fluorescent qtlantitive PCR（qRT-PCR） was used to detect the expression of miR-218 in HCC and control tissues. The relationship between miR-218 expression and HCC clinicopathological parameters was analyzed. [Results] Compared with the control tissues, the expression of miR-218 in HCC tissue was consistently down-regulated, and there was significant difference（ P d0.01）. The average expression level of miR-218 in control tissues was 2.90-fold of HCC tissue. The expression level of miR-218 was related with HCC malignancy, tumor differentiation, intrahepatic and extrahepatic metastasis and portal vein embolus. There was no correlation between miR-338 expression and the age, sex, alcohol history, HBsAg, tumor size and the combination with liver cirrhosis of HCC patients. [Conclusion] MiR-218 is closely associated with the carcinogenesis and clinical progression of HCC. MiR-218 may be a new potential therapy target and molecule marker of the diagnosis and prognosis of HCC.