脑钠尿肽基因rs198389单核苷酸多态性与冠心病的相关性研究

Association between single nucleotide polymorphism rs198389 in brain natriuretic peptide gene and coronary heart disease.

目的探讨脑钠尿肽（BNP）基因rs198389位点单核苷酸多态性（SNP）与冠心病（CHD）的关系。方法取病例-对照方法，收集冠心病和健康者各961例，应用Real-timeqPCR（TaqMan探针）技术进行基因分型，分析BNP基因rs198389位点SNP与CHD危险性的关系。结果CHD组和对照组比较，AA和GG基因型、A等位基因和G等位基因分布差异有统计学意义（P〈0．05）；GG基因型和G等位基因频率在病例组（3．6％和16．7％）明显高于对照组（2．1％和14.3％）。与A等位基因比较，G等位基因使CHD危险性显著增加（OR=1．20，95％CI=1．01-1．43，P=0．04）。GG基因型者CHD危险性增加（P=0．04），但在调整年龄、性别、吸烟、饮酒、体重指数、血甘油三脂、高血压史、糖尿病史和CHD家族史等变量后结果差异无统计学意义（P=0．12）。结论BNP基因位点rs198389的G等位基因使CHD危险性显著增加；BNP基因rs198389位点SNP可能与CHD易感性相关，但不是其独立危险因素。

Objective To investigate the association between single nucleotide polymorphism rs198389 in-brain natriuretic peptide （BNP） gene and coronary heart disease （CHD）. Methods Patients with CHD （the CHD group, n=961） and 961 healthy subjects （n=961, the control group） were enrolled in this study. The BNP rs198389 polymorphism was genotyped by real-time quantitative polymerase chain reaction （Real-time qPCR）. The association between BNP rs198389 polymorphism and CHD was analyzed by SPSSI0.0 software. Results There was statistical- ly significant difference in the distribution of AA and GG genotypes between the CHD group and the control group （P〈 0.05）. The frequencies ofGG genotype and G allele were significantly higher in CHD group （3.6% and 16.7%） than in the control group （2.1% and 14.3%）. G allele carries significantly higher risk of CHD than A allele （P=0.04）. GG geno- type presented a significantly higher risk of CHD （P=-0.04）, but this risk was not more significant when adjusted for CHD risk factors by unconditional Logistic regression analysis （P=0.12）. Conclusion The G allele of BNP gene rs198389 site significantly increased the risk of CHD. The results suggest that BNP gene rs198389 may be a suscepti- bility gene of CHD, but it is not independent risk factor.