肌酐代谢产物对肾小管上皮细胞凋亡的影响

Effect of metabolites of creatinine on the apoptosis of renal tubular epithelial cells.

目的研究肌酐产物是否能促进肾小管上皮细胞凋亡。方法原代培养人肾小管上皮细胞,将肌酐产物与肾小管上皮细胞共同培养,对肾小管上皮细胞进行形态学观察;抽提DNA进行琼脂糖电泳观察有无梯形条带。结果肾小管上皮细胞在肌酐产物作用下逐渐变小、变圆、固缩,最后漂浮死亡,但胞膜始终完整;肌酐产物导致肾小管上皮细胞凋亡,琼脂糖凝胶中有DNA梯形条带,加入谷胱甘肽(GSH)未见DNA梯形条带。结论肌酐产物促进肾小管上皮细胞凋亡,GSH可阻断之。

Objective To study the effect of metabolites of creatinine on the apoptosis of renal tubular epi- thelial cells. Methods Renal tubular epithelia were cultured in vitro. The metabolites of creatinine and renal tubular epithelial cells were incubated together. The morphological change of renal tubular epithelial cells was observed. Gel electrophoresis of DNA extracted from that to observe bands of apoptotsis. Results Affected by metabolites of creati- nine, renal tubular epithelial cells showed characters of apoptosis （smaller, round, pyknosis and death）, but the cell membrane was always integral. Agarose gel electrophoresis revealed the appearance of DNA ladder, which disap- peared with the addition of GSH. Conclusion The metabolites of creatinine can induce the apoptosis of renal tubular epithelial cells, which could be reversed by GSH.