细菌性脑膜炎患儿脑脊液和血清中TNF-α和IL-1β的表达及其临床意义

Expressions and significance of TNF-α and IL-1β in the serum and cerebrospinal fluid of children with bacterial meningitis.

目的观察急性期细菌性脑膜炎(BM)和病毒性脑膜炎(VM)患儿血清和脑脊液中肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)的水平变化,探讨其临床意义。方法收集在本院治疗的BM患儿22例、VM患儿18例的急性期脑脊液和血清。采用双抗体酶联免疫吸附法(ELISA)检测两组对象血清和脑脊液中TNF-α和IL-1β的表达水平。结果 BM组脑脊液TNF-α及IL-1β水平明显高于VM组(P<0.05),BM组血清TNF-α水平明显高于VM组(P<0.05),BM组和VM组血清IL-1β的变化差异无统计学意义(P>0.05)。BM脑脊液TNF-α及IL-1β水平呈明显正相关(r=0.635)。结论脑脊液和血清TNF-α与IL-1β的联合检测可以用于对BM患儿的鉴别诊断。

Objective To evaluate the levels of TNF-ct and IL-1β in the serum and cerebrospinal fluid of children with bacterial meningitis （BM） and those with virus meningitis （VM）. Methods Serum and cerebrospinal fluid samples were collected from 22 children with BM （BM group） and 18 children with VM （VM group）. The levels of TNF-α and IL-1β in the samples were evaluated by ELISA. Results The levels of B7-H3 and IL-1β in cerebro- spinal fluid of BM group were significantly higher than those of VM group （P〈0.05）. The levels of TNF-α in serum of BM group was significantly higher than that of VM group （P〈0.05）. The levels of IL-1β in serum of BM and VM group showed no statistically significant difference （P〉0.05）. The levels of TNF-α and IL-1β in cerebrospinal fluid of BM group were positively correlated （r=0635, P〈0.05）. Conclusion Combined test of TNF-α and IL-1β could be used for the differential diagnosis of children with bacterial meningitis.