炎性因子的表达与骨-肌腱结点损伤的相关性研究

Expression of inflammatory factors and its correlation with bone-tendon junction injury

目的探讨IL-1、IL-6及IL-17表达与骨-肌腱结点愈合之间的相关性。方法随机选取36只成年新西兰大白兔作为实验对象,所有新西兰白兔分别于术后2周、4周、8周取材。实验动物行组织学检测和双抗体夹心ABC-ELISA法检测。结果术后2周、术后4周实验动物血清中IL-1、IL-6及IL-17的表达显示升高,其表达水平与术前比较差异有统计学意义(P<0.05)。术后8周实验动物血清中IL-1、IL-6及IL-17的表达与术前比较差异无统计学意义(P>0.05)。结论炎性因子IL-1、IL-6及IL-17与骨-肌腱结点愈合之前相关性极高,研究其相关性在结点病治疗中有着重要的意义。

Objective To investigate the correlation between the expression of IL-1, IL-6 and IL-17 and bone-tendon junction injury. Methods Thirty-six adult New Zealand rabbits were selected as experimental subjects. Samples were taken 2, 4, 8 weeks after operation for histological detection and double antibody sandwich ABC-ELI- SA. Results The levels of IL-1, IL-6 and IL-17 increased sigaificantly 2, 4 weeks after operation, compared with before operation （P〈0.05）. The levels of IL-1, IL-6 and IL-17 eight weeks after operation were not significantly dif- ferent from that before operation （P〉0.05）. Conclusion Inflammatory factors （IL-1, IL-6 and IL-17） are closely cor- related with bone tendon junction healing.