基于pH恒定补糖的生物基丁二酸高效合成

Enhanced bio-succinic acid synthesis based on glucose-fed with pH-constant

通过确定大肠杆菌在产酸阶段葡萄糖的消耗与酸中和剂碳酸钠间的定量关系,建立了pH恒定补糖策略,能够使发酵液中葡萄糖浓度维持在稳定水平。与分批发酵相比,采用pH恒定补糖且维持葡萄糖浓度在较低的水平对丁二酸的积累是有利的。当采用pH恒定补糖并控制葡萄糖质量浓度在10g/L时,丁二酸最终质量浓度达到57.6 g/L,生产效率达到1.15 g/(L·h)。

A strategy based on pH-constant is established by investigating the ratio of glucose to Naz CO3 consumption during the succinic acid production stage. This method can maintain the glucose concentration at a constant level. Compared with the batch fer- mentation, it is favourable for succinic acid accumulation when pH-constant fermentation is adopted and glucose concentration is main- tained at a low level. In the case of the glucose fed-batch fermentation with pH-constant and controlling the glucose mass concentration at 10 g/L, the succinic acid titer and productivity reaches 57.6 g/L and 1.15 g/（ L·h）, respectively.