EPO对AD样大鼠海马组织氧化应激反应的影响

Effects of EPO on oxidative stress in Hippocampus of Rats with Alzheimer's disease

目的观察EPO对Alzheimer样大鼠总抗氧化能力、过氧化氢酶活性、一氧化氮含量及一氧化氮合酶活性的影响。方法 SD大鼠随机分为生理盐水组、AD模型组、EPO治疗组。采用淀粉样蛋白β(Aβ)直接海马注射建造阿尔茨海默病(AD)模,生理盐水组海马注射等量生理盐水,EPO治疗组从造模后第1起腹腔注射浓度为5000IU/kg的重组人促红细胞生成素(rHu-EPO)治疗10天(2天1次)。并应用分光光度法测定大鼠海马组织T—AOC、CAT活性、NO含量及NOS活性。结果与生理盐水组和EPO治疗组相比,AD模型组的T—AOC和CAT活性明显降低(P<0.05),NO含量及NOS活性明显升高(P<0.05)。结论 EPO能够提高AD样大鼠海马组织的抗氧化能力,明显地减轻氧化应激反应对AD样大鼠海马组织的损害。

Objective To explore the effect of EPO on T--AOC CATNO and NOS in Hippocampus of Alzheimer＇ s disease Rats. Methods Spraque-Dawley rats were randomly separated into 3 groups：saline group, AD model group and EPO-treatment group. With amlyoid beta-protein（Aβ）injection into rat hippocampus made Alzheimer＇ s disease model. Hippocampus of saline group injected normal saline. EPO-treatment group was treated 10 days（ 1 times 2 days） by intraperitoneal injection of Concentration for 5 000 iu/kg of rHu-EPO from the first day after the modeling. T--AOC^CAT^NO and NOS in rats＇ hip- pocampus were assayed by spectrophotometry. Results Compared to the saline group and the EPO - treatment group, the ability of T--AOC and the activity of CAT in rats＇ hippocampus of AD model group were decreased （ P 〈0. 05 ） , but the contents of NO and the activity of NOS were increased in AD model group （P 〈 0.05）. Conclusion EPO may raise the anti-oxidant ability of hippoeampus tissue of Alzhei- mer＇ s disease Rats, significantly decrease injury of oxidative stress in hippoeampus of rats with Alzbei- mer＇ s disease.