加味二至丸对卵巢切除小鼠的影响

Effects of Modified Erzhi Pills on Ovariectomized Mice

【目的】观察加味二至丸对卵巢切除小鼠血脂4项、糖耐量及腹腔内脂肪细胞核中p65蛋白表达水平的影响。【方法】32只雌性昆明种小鼠分为假手术组,卵巢切除模型组,加味二至丸(中药)高、低剂量组(剂量分别为0.8、0.2 g.kg-1.d-1)。动物灌胃16周后,测定各组小鼠血脂4项及糖耐量水平。应用免疫荧光法检测小鼠内脏脂肪组织胞核中p65蛋白表达水平。【结果】中药高、低剂量组血清甘油三酯水平较模型组显著降低(P<0.05),30、60、90 min时间点血糖水平显著降低(P<0.05),糖耐量曲线下面积较模型组均显著降低(P<0.05);免疫荧光显示模型组小鼠脂肪组织胞核中p65蛋白表达水平显著升高,中药高、低剂量组可显著抑制p65蛋白表达水平(P<0.05)。【结论】加味二至丸可降低更年期模型小鼠血清甘油三酯水平并改善小鼠的糖耐量,这可能与加味二至丸降低脂肪组织胞核中p65蛋白表达有关。

Objective To investigate the effects of modified Erzhi Pills （MEP） on plasma four-item lipids,glucose tolerance and intraabdominal adipocyte nuclear p65 protein level in ovariectomized Kunming mice. Methods Thirty-two female Kunming mice were divided into four groups, sham operation group, ovariectomized model group, and high-and low-dose MEP groups （in the dosage of 0.8, 0.2 g.kg-1.d-1 respectively） . After treatment for sixteen weeks, the levels of serum lipid levels and glucose tolerance in four groups were determined. The level of nuclear p65 protein in intraabdominal adipocytes was determined by immunofluorescence assay. Results The serum triglyceride level as well as serum glucose level at time point of 30, 60 and 90 min was decreased in high- and low-dose MEP groups, and the area under the curve in glucose tolerance test was also reduced （P〈0.05 compared with those in the ovariectomized model group） . The results of immunofluorescence assay showed that nuclear p65 protein level was significantly increased in ovariectomized model mice, and was decreased in high- and low-dose MEP groups （P〈0.05 compared with that in the ovariectomized model group）. Conclusion Modified Erzhi Pills shows obvious effect on decreasing serum triglyceride level and on improving glucose tolerance, which is probably related with the decrease of nuclear p65 protein levels in intraabdominal adipocytes.