冠通方及其拆方含药血清对大鼠血管平滑肌细胞C-myc癌基因表达的影响

Effect of Serum Containing Guantong Decoction and Its Separate Prescriptions on C-myc Gene Expression in Rat Vascular Smooth Muscle Cells

【目的】观察冠通方及其拆方含药血清对血管紧张素Ⅱ(AngⅡ)诱导的大鼠血管平滑肌细胞(VSMC)C-myc癌基因表达的影响。【方法】选用大鼠VSMC,采用AngⅡ诱导其增殖,以冠通方及其拆方含药血清分别作用于大鼠VSMC,采用逆转录—聚合酶链反应(RT-PCR)方法,测定冠通方及拆方含药血清对AngⅡ诱导的VSMC C-myc癌基因表达的情况。【结果】AngⅡ组VSMC的C-myc/β-actin比值显著增加,与空白对照组比较差异有统计学意义(P<0.01);冠通方组及其拆方各组均可使C-myc比值显著下降,与AngⅡ组比较差异均有统计学意义(P<0.01);冠通方组与其拆方各组比较差异均有统计学意义(P<0.05或P<0.01);各拆方组组间比较差异均无统计学意义(P>0.05)。【结论】冠通方抑制VSMC增殖的作用与其可下调VSMC的调控基因C-myc表达有关,且冠通方组作用优于其拆方组。

Objective To observe the effect of serum containing Guantong Decoction and its separate prescriptions on C-myc gene expression in rat vascular smooth muscle cells （VSMCs） stimulated by angiotensinⅡ （Ang Ⅱ ）. Methods The rat VSMCs were stimulated by Ang Ⅱand were treated by serum containing Guantong decoction and its separate prescriptions. C-myc gene expression was determined by reverse-transcription polymerase chain reaction （PCR） method. Results The C-myc/β-actin ratio in the Ang Ⅱ stimulation group was increased, the difference being significant compared with that in the blank control group （P〈0.01）. The C-myc/β-actin ratio in Guantong Decoction group and three separate prescriptions groups was decreased, the difference being significant compared with that in Ang Ⅱ stimulation group （P〈0.01）. Guantong Decoction group had lower c-myc/β-actin ratio than the three separate prescription groups （P〈0.05 or P〈0.01）, but the differences were insignificant among three separate prescription groups （P〉0.05）. Conclusion Inhibition of VSMCs proliferation by Guantong Decoction is probably related with down-regulation of C-myc gene expression, and Guantong Decoction has better inhibitory effect than the separate prescriptions.