小花棘豆生物碱对小白鼠外周血T淋巴细胞亚群CD_4^+、CD_8^+免疫功能的影响

Effects of Oxytropis glabra Alkaloids on Mice Peripheral Blood T Lymphocyte Subsets CD_4^+ and CD_8^+ Immune Function

试验采用小花棘豆生物碱苦马豆素(SW)、黄花碱(Ts)以及2种生物碱的混合物分别对小白鼠进行传统灌胃,并于试验开始后第7天、第14天、第21天、第28天、第35天,从各组中随机抽取4只小白鼠,自其眼球取抗凝血,利用流式细胞术检测外周血T淋巴细胞亚群CD4+、CD8+的百分检率,并计算出CD4+与CD8+的比值。结果表明,低浓度的SW具有提高机体免疫机能的作用,而中等浓度和高浓度的SW在短时间内仍然可以提高机体的免疫机能,长时间使用却会严重降低机体的免疫机能;Ts在低浓度下不会影响机体的免疫机能,中等浓度和高浓度能够降低机体的免疫机能;混合生物碱组在低浓度时对机体的免疫机能影响不大,但在高浓度时仍然能够显著降低机体的免疫机能。

Three groups of mice were given with swainsonine （SW）, thermopsine （Ts） and their mixtures by gavage, respectively Four mice from each group were randomly chosen to take the anticoagulant blood of the eyes after 7, 14, 21, 28 and 35 days respectively. The peripheral blood T cell subsets CD4＋ and CD8＋ were assayed by flow cytometry, and the ratio of CD4＋ to CD8＋ wa calculated. The results showed that the low dose of SW could play a significant role in enhancing immunity of mice, while th, medium and high concentration SW played an active role in short time, but decreased immunity along with the extension of time the low concentration of Ts didn＇t work on immunity in mice, while the medium and high concentration of Ts could reduc, immunity; the low concentration of mixtures had little effect on the immunity of mice, while the high concentration of mixture could still decrease immunity significantly.