聚乙烯醇/壳聚糖多孔水凝胶的制备和性能研究

Preparation and Properties of PVA/CS Porous Hydrogen

目的制备一种可用于软骨修复的水凝胶材料。方法 以聚乙烯醇(PVA)、壳聚糖(CS)以及聚山梨酯-80(Tween-80)为原料,通过乳化发泡-冷冻冰晶相分离-去除表面活性剂法制备了PVA/CS多孔水凝胶,并采用IR、SEM、DSC、与MG63复合培养法及MTT法检测材料性能和细胞相容性。结果 CS分子与PVA分子有很强的氢键作用,对CS的分子C-O键伸缩振动和-OH弯曲振动产生影响,使其特征峰向高波数移动;与未加入乳化剂的材料相比,加入乳化剂处理的凝胶表面和内部存在不同尺寸和形状的孔洞和索状结构,并在大孔周围分布有微孔,且孔与孔之间贯通性好,为水凝胶提供了良好的可渗透性。当PVA与CS共混后,熔融峰归结于PVA的结晶熔融峰。与MG63复合培养结果显示PVA/CS多孔水凝胶具有较低的毒性,较好的细胞生长及增殖行为。结论 PVA/CS多孔水凝胶具有较好的材料学及生物学性能,在软骨修复方面具有潜在的应用前景。

Objective To prepare PVA/CS porous hydrogen designed for cartilage defect repair. Method PVA/CS porous hydrogen was prepared by polyvinyl alcohol （PVA） , chitosan （CS） , and Polysorbate- 80 （Tween- 80）. The hy- drogen preparation process consisted of emulsifier - foaming, freeze - drying and surfactant - cleaning. The porous hydro- gen were characterized by infrared spectroscopy （IR）, scan electron microscopy （SEM）, differential scanning calorimeter （ DSC）, further cultured with MC,63 and by using MTT assays. Result The crystal of the CS was disrupted by strong hy- drogen bonds between the CS molecules and the PVA molecules, the C - 0 stretching vibration and the - OH bending vi- bration of CS molecular were changed, causing the characteristic adsorption peaks of the CS shifting towards higher wave numbers. With the addition of emulsifier, the structure of PVA/CS gel was changed. The interconnected pores with differ- ent sizes and shapes appeared, providing good permeability. After blending PVA with CS, the melting peak of the compos- ite hydrogen was attributed to the melting peak of the pure PVA. The SEM images and MTr assays proved all the hydrogen were of low toxicity, good cell growth and proliferation. Conclusion PVA/CS porous hydrogen with its good material property as well as good biocompatibility has promising future in cartilage defect repair.