胰高血糖素样肽-1对高糖培养肾小球系膜细胞氧化应激的影响被引量：1

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摘要目的探讨胰高血糖素样肽-1(glucagon like peptide-1,GLP-1)对高糖所致大鼠肾小球系膜细胞氧化应激的影响。方法肾小球系膜细胞按下列分组培养:正常对照组(5.5mmol/L),高糖组(25mmol/L),高糖+GLP-1(3nmol/L)组,高糖+GLP-1(10nmol/L)组,高糖+GLP-1(30nmol/L)组,高糖+GLP-1(100nmol/L)组,分别培养24h后,采用CCK8试剂盒检测细胞增殖率,流式细胞仪法检测细胞活性氧(ROS),酶标仪法检测细胞上清液中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及还原型谷胱甘肽(GSH)含量。结果高糖培养组肾小球系膜细胞增殖,ROS生成增多,SOD及GSH活性下降,MDA含量升高,GLP-1(3nmol/L)干预对上述改变无影响,10、30、100nmol/L GLP-1干预均可拮抗高糖培养时系膜细胞的上述改变,其中100nmol/L GLP-1干预效果最为显著。结论 GLP-1对高糖所致肾小球系膜细胞的氧化应激反应呈现浓度依赖性的抑制作用。

作者孙靖陈容平杨锐张振陈思蔡德鸿陈宏

机构地区南方医科大学珠江医院内分泌科南方医科大学

出处《广东医学》 CAS CSCD 北大核心 2013年第7期1008-1010,共3页 Guangdong Medical Journal

关键词 GLP-1 高糖肾小球系膜细胞氧化应激

分类号 R587.24 [医药卫生—内分泌]

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