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过氧化物酶体增殖物激活受体γ抑制高糖诱导的肾小管上皮细胞转分化 被引量：2

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摘要 目的观察过氧化物酶体增殖物激活受体γ(PPARγ)在高糖诱导的肾小管上皮细胞转分化中的作用。方法大鼠肾小管上皮细胞随机分成3组:对照组(C组)、高糖组(D组)和PPARγ激动剂组(R组)。C组加正常培养液,D组在培养液中加30mmol/L葡萄糖,R组在含30mmol/L葡萄糖的培养液中加入20μmol/L的罗格列酮。在处理后6、12、24、48、72 h时用免疫细胞化学、Western blot检测上皮细胞标志物E-钙黏素(E-cadher-in)和肌成纤维细胞标志物α-平滑肌肌动蛋白(α-SMA)及PPARγ的表达。结果 C组各时间段均无α-SMA的阳性表达,而E-cadherin和PPARγ呈强阳性表达;D组12 h时出现α-SMA阳性表达,48 h达高峰并持续至72 h,而E-cadherin和PPARγ在6 h出现下降,并持续至72 h;R组各时间段均只有少数α-SMA阳性细胞,与D组比较,PPARγ在6 h出现升高,并持续至72 h。结论上调PPARγ可抑制高糖诱导大鼠肾小管上皮细胞转分化。
作者 黄晓霞 柯昌斌
机构地区 广东省深圳市宝安区人民医院肾内科 湖北医药学院附属太和医院肾内科 广东省深圳市宝安区人民医院麻醉科 湖北医药学院附属太和医院麻醉科
出处 《广东医学》 CAS CSCD 北大核心 2013年第7期1017-1021,共5页 Guangdong Medical Journal
关键词 过氧化物酶体增殖物激活受体Γ 肾小管上皮细胞 转分化 高糖
分类号 R541.4 [医药卫生—心血管疾病]
  • 相关文献

参考文献14

  • 1TAKEJI M, KAWADA N, MORIYAMA T, et al. CCAAT/Enhan- cer- binding protein contributes to myofibroblast transdifferentia- tion and renal disease progression [ J ]. J Am Soc Nephrol, 2004, 15(9) : 2383 -2390.
  • 2MASSZI A, FAN L, ROSIVALL L, et al. Integrity of cell - cell contacts is a critical regulator of TGF - beta 1 - induced epithelial - to -myofibroblast transition: role for beta- catenin[ J]. J Am Pa- thology, 2004, 165(6): 1955-1967.
  • 3HATANAKA H, KOIZUMI N, OKUMURA N, et al. Epithelial - Mesenchymal transition - like phenotypic changes of retinal pig- ment epithelium induced by TGF - β are prevented by PPAR - γ, agonists [ J ]. Invest Ophthalmol Vis Sci, 2012, 53 (11 ) : 6955 - 6963.
  • 4CHEN L, NECELA B M, SU W, et al. Peroxisome proliferator- activated receptor 3, promotes epithelial to mesenchymal transforma- tion by Rhn GTPase- dependent activation of ERK1/2 [ J]. J Biol Chem, 2006, 281 (34): 24575-24587.
  • 5AIELLO A, PANDINI G, FRASCA F, et al. Peroxisomal prolife- rator - activated receptor - gamma agonists induce partial reversion of epithelial- mesenchymal transition in anaplastie thyroid cancer cells [ J ]. Endocrinology, 2006, 147 (9) : 4463 - 4475.
  • 6ZHANG A H, DONG Z, YANG T X. Prostaglandin D2 inhibits TGF- β1 - induced epithelial - to - mesenchymal transition in MDCK cells[ J]. Am J Physiol Renal Physiol, 2006, 291 (6): 1332 - 1342.
  • 7刘鸣,王少华,李月白,等.靶向PPAR基因siRNA腺病毒对乙醇抑制家兔骨髓基质细胞成骨分化的影响[J].郑州大学学报:医学版,2009,44(2):269-272.
  • 8张莉莉,李敬诚,王景周,谢鹏.PPAR-γ在高血压血管平滑肌细胞表型转化中的作用[J].第三军医大学学报,2009,31(4):311-314. 被引量：6
  • 9TAKEJI M, KAWADA N, MORIYAMA T, et al. CCAAT/Enhan- eer- binding protein delta contributes to myofibroblast transdiffe- rentiation and renal disease progression[ J]. J Am Soc Nephrol, 2004, 15(9) : 2383 -2390.
  • 10YANG Jun - wei, DAI Chun - sun, LIU You - hua. /-Iepatocyte growth factor suppresses renal interstitial myofibroblast activation and intercepts Smad signal transduetion [J]. J Am Pathology, 2003, 163(2) : 621 -632.

二级参考文献8

  • 1张莉莉,祝之明,曹廷兵,王利娟.Blockage of PPARδ increases the expression of inflammatory factors in 3T3-L1 cells stimulated with TNFα[J].Journal of Medical Colleges of PLA(China),2006,21(2):77-81. 被引量：2
  • 2Kon K, Ikejima K, Hirose M, et al. Pioglitazone prevents early-phase hepatic fibrogenesis caused by carbon retrachloride. Biochem Bilphys Res Commun, 2002 ,291(1):55-61
  • 3Marra F, Efsen E, Romanelli RG,et al. Ligands of peroxisome proliferator-activated receptor gamma modulate profibrogenic and proinflammatory actions in hepatic stellate cells. Gastroenterology, 2000,119(2): 466～478
  • 4Braissant O, Foufelle F, Scotto C, et al. Differential expression of peroxisome proliferator-activated receptors(PPARs):tissue distribution of PPAR-α,- β,and -γin the adult rat.Endocrinology ,1996,137(1):354-366
  • 5Everett L, Galli A, Grabb D . The role of hepatic peroxisome proliferator -activated receptors (PPARs) in health and disease. Liver,2000 ,20(3): 191-199
  • 6Hashimoto T, Cook WS, Gi C, et al. Defect in peroxisome proliferator-activated receptor alpha-inducible fatty acid oxidation determines the severity of hepatic steatosis in response to fasting .J Biol Chem,2001,275(37):28918～28928
  • 7Kersten S,Seydoux J,Peters JM,et al. peroxisome proliferator-activated receptor alpha mediates the adaptive response to fasting. J Clin Invest,1999,103(111):1489-1498
  • 8Reginato MJ, Krakow SL,Bailey ST,et al. Prostaglandins promote and block adipogenesis through opposing effects on peroxisome proliferator-activated receptor gamma. J Biol Chem,1998,273(22):1855-1858

共引文献11

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二级引证文献10

广东医学
2013年 第7期

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