寒淫致病及附子干预的转录组生物学网络构建研究

Biology Net-Work of Transcriptome in Cold Pathogen and Radix Aconiti Lateralis Preparata Intervention

目的:基于生物学网络构建分析"寒淫"致病及附子干预潜在的生物学标志物。方法:实验动物暴露于(-10±2)℃的环境中21 d,采用附子作为干预药品,实验完成后提取股动脉血进行全基因组扫描,对芯片数据采用生物网络构建方法进行网络构建。结果:"寒淫"致病有关的关键生物学过程主要有细胞外基质受体相互作用、细胞因子相互作用、黏着斑通路、P53信号通路、细胞周期、抗原加工提呈与内吞作用。附子干预有关的关键生物学过程主要是细胞外基质受体相互作用、细胞因子相互作用、细胞黏附分子、钙信号通路、P53信号通路、细胞周期与抗原加工递呈。结论:生物学网络构建在中医"寒淫"研究中发挥重要作用,比之前的孤立变化基因分析更为准确的体现分子相互作用关系。生物学网络构建为中医证候研究提供了一个有效的信息分析方法。

Objective ： The aim of this study was to investigate potential biology net - work for cold pathogen and radix aconiti lateralis preparata intervention. Methods：Experimental animals were exposed to （ -10 ± 2） ℃environment for 21 days and inter- vened by radix aconiti lateralis preparata. Blood was collected after experiment for the whole genome, and biology net - work was used to analyze microarray datum. Results ： The key biological process of cold pathogen is ECM - receptor interaction, cytokine - cytokine receptor interaction, focal adhesion, p53 signaling pathway, cell cycle, antigen processing and presentation. The key bio- logical process of radix aconiti lateralis preparata intervention is ECM - receptor interaction, cytokine - cytokine receptor interac- tion, cell adhesion molecules, calcium signaling pathway, p53 signaling pathway, cell cycle and antigen processing and presenta- tion. Conclusion ： Biology network plays an important role in the study of cold pathogen in TCM and can provide a better exact un- derstanding for the molecular interaction relations. Biology network provides an effective information analysis method for the syn- drome of TCM.