花生枝叶治疗HHcy致家兔动脉粥样硬化的实验研究

Effect of Huashengzhiye on Atherosclerosis Caused by Hyperhomocysteinemia

目的:探讨花生枝叶对动脉粥样硬化(AS)家兔的干预效果。方法:28只新西兰白兔随机分成两组,其中23只通过注射DL-蛋氨酸造模,5只作正常对照组。4周后剔除造模不成功者后,将造模成功家兔随机分成3组:模型组、辛伐他汀组、花生枝叶组。第5周时,花生枝叶组给予花生枝叶水煎剂灌胃(3.96 g/kg),辛伐他汀组给予辛伐他汀灌胃(5 mg/kg),正常对照组和模型组给予蒸馏水灌胃3 mL,均每日两次。分别在第0、4、8、12周末时检测血清同型半胱氨酸(Hcy)水平和B超检测血管内中膜厚度阻力指数等,12周末处死家兔,HE法检测病理学改变。结果:与正常对照组比较,模型组各时间点Hcy明显升高(P<0.05),内中膜厚度明显增厚(P<0.05),HE染色显示内膜明显增厚,花生枝叶组和辛伐他汀组在各时间点能够不同程度地降低Hcy,减小内中膜厚度(P<0.05),HE染色显示内膜增厚不明显。结论:花生枝叶能降低Hcy,抑制斑块的增长,具有治疗AS的作用。

Objective ：To observe the effect of Huashengzhiye on atherosclerosis. Methods：Twenty-eight female New Zealand white rabbits were randomly divided into two groups, one group （5 rabbits）was for controlling, the other one （23 rabbits） was for modeling. 4 weeks later, the succeeded model were divided into 3 groups ： the model group, the Simvastatin group and the Huash- engzhiye group. From the begining of 5th week ,rabbits were given Simvastatin pills to the simvastatin group （5 mg/kg） and Hua- shengzhiye to the Huashengzhiye group （3.96 g/kg） respectively twice a day and lasted eight weeks. 3 mL distilled water was giv- en to the control group and model group. The Hey in the serum and Intima-media thickness were detected respectively at the end of 0w.4w.8w.12w. At the end of 12w.vaseular pathological changes were observed by the methods of HE stain. Results ：Comparedwith the control group, the level of Hcy in serum of the model group was significantly improved, the intima-media thickness was in- creased. Compared with the model group, the levels of Hcy in serum of the Simvastatin group and the Huashengzhiye group were significantly lower,the intima-media thickness was lessen,and the HE stain showed that the intima was less incrassated. Conclu- sion ： Huashengzhiye could decrease the level of Hcy,inhibit the thickening of plaque, and it has the effect of anti-atherosclerosis.