摘要
目的观察柿叶黄酮(PLF)对阿霉素肾病大鼠肾组织金属蛋白酶抑制物1(TIMP-1)表达及对肾组织病理损伤的影响。方法制备阿霉素肾病大鼠模型及药物干预模型鼠,测定模型鼠24 h尿蛋白(24hUP)变化,用免疫组化法检测肾组织中TIMP-1表达,光学显微镜观察肾组织病理形态。结果 PLF能明显降低阿霉素肾病大鼠24hUP(P<0.01),减轻肾脏免疫病理损伤及抑制肾组织TIMP-1的表达(P<0.01)。结论 PLF能降低模型鼠肾组织TIMP-1的表达、抑制肾组织纤维化等,对阿霉素肾病大鼠肾脏起到保护作用。
Objective To observe the effect of persimmon leaf flavonid（PLF） on the expression of TIMP-1 in kidney of adriamycin nephropathy（AN） rat.Methods AN rat model and drug intervention rat model were prepared.The expression of TIMP-1 was detected with immunohistochemical method.The pathomorphological change of kidneys was detected by microscope.24-h urine protein（24hUP） was detected in different times.Results PLF could obviously reduce 24hUP,improve pathologic damage of kidneys and inhibit the expression of TIMP-1 in kidneys of AN rats（P〈0.01）.Conclusion PLF can protect the kidneys of AN rats by down regulating expression of TIMP-1 and inhibiting tissue fibrosis,etc.
出处
《现代医药卫生》
2013年第9期1283-1284,1287,共3页
Journal of Modern Medicine & Health
基金
2011广西壮族自治区教育厅科研基金资助项目(200103YB155)
关键词
黄酮
分离和提纯
柿树
化学
疾病模型
动物
肾疾病
化学诱导
金属蛋白酶1组织抑制剂
阿霉素肾病
24h尿蛋白
Flavone/isolation ＆ purification
Diospyros kaki/chemistry
Disease models
animal
Kidney diseases/chemically induced
Tissue inhibitor of metalloproteinase-1
Adriamycin nephropathy
24-h urine protein
