H_2O_2致小鼠淋巴细胞DNA的损伤及修复

Experimental study of DNA damage induced by H_2O_2 in lymphocytes

目的 应用单细胞电泳检测 (SCGE)过氧化氢 (H2 O2 )致小鼠淋巴细胞 DNA损伤及其修复。方法 标准 SCGE条件下荧光显微镜观察。结果 H2 O2 不同浓度 80、16 0和 32 0μmol/ L ,3个组 H2 O2 浓度均可致小鼠淋巴细胞 DNA损伤 ,较低剂量即可造成细胞拖尾 ,可逆性损伤在 6 0 m in内可获得明显修复。结论 单细胞电泳可快速灵敏地检测氧化损伤 ,也可能用于流行病学研究。

Objective To monitor DNA damages and repairs in lymphocytes.Method Single cell gel elecrophoresis (SCGE) assay was used.Result The study showed that H_2O_2 caused DNA damage which could also be repaired in lymphocytes.Conclusions SCGE was a fast and sensitive method to detect cell oxidative damage caused by H_2O_2.The assay may be also useful in molecular epidemiology.