小鼠子宫腺肌病模型病灶中雌孕激素受体的表达及意义

Expression of estrogen receptor and progesterone receptor in mice adenomyosis model

目的探讨雌激素受体(ER)和孕激素受体(PR)在子宫腺肌病模型病灶中的表达及临床意义。方法用异体垂体移植法建立ICR小鼠子宫腺肌病模型,应用免疫组化SP法检测正常子宫内膜、模型子宫内膜及病灶异位子宫内膜中ER、PR的表达。结果垂体移植4个月和6个月后,均成功诱发子宫腺肌病模型,不同时间诱发模型的病灶大体结节数和HE染色评分之间比较,均无统计学差异(均P>0.05);建模6个月后模型病灶的结节显著大于建模4个月后(P<0.05)。建模后4个月,模型子宫内膜和病灶异位子宫内膜以及正常子宫内膜中ER和PR表达的阳性率比较。均无统计学差异(均P>0.05);建模后6个月,3种子宫内膜中ER阳性率分别为55.07%、31.63%和24.74%;PR阳性率为48.40%、27.48%和22.70%;3者比较均有统计学差异(均P<0.05)。结论子宫腺肌病的发生、发展可能与子宫内膜局部ER、PR的高表达相关。

Objective To investigate the expression estrogen receptor （ER） and progesterone receptor （PR） in rice ade-nomyosis model and its significance. Methods The adenomyosis model was induced by al ogeneic pituitary transplantation in I-CR mice. The expression of ER and PR in endometrial tissue was detected by immunohistochemistry SP method. Results Ade-nomyosis was induced successful y by allogeneic pituitary transplantation in ICR mice 4 months and 6 months after transplanta-tion. There were no differences in the number of nodules and the grades of H＆E staining between 4 and 6 months after transplan-tation （P〉0.05）. But the size of nodules at 6 months was larger than that at 4 months （P〈0.05）. At 6 months after transplantation, the positive rate of ER and PR in endometrial tissue or ectopic endometrial tissue were higher than that in normal endometrial tis-sue （P〈0.05）;however, the difference was not observed at 4 months after transplantation. Conclusion The expression of ER and PR in endometrial tissue is associated with the development of adenomyosis in ICR mice model.