β-榄香烯诱导人骨肉瘤MG-63细胞凋亡作用

Effects of β-elemene on apoptosis of human osteosarcoma MG-63 cells

[目的]观察β-榄香烯诱导人骨肉瘤MG-63细胞的凋亡作用,并探讨其可能的机制.[方法]将不同质量浓度的β-榄香烯作用于人骨肉瘤MG-63细胞,用MTT法测定细胞存活率,流式细胞术测定细胞凋亡,免疫荧光染色、蛋白印迹检测凋亡相关蛋白的表达.[结果]与对照组比较,β-榄香烯处理组细胞存活率明显降低(P<0.05);凋亡相关蛋白表达明显增加(P<0.01).[结论]β-榄香烯可诱导人骨肉瘤MG-63细胞的凋亡,β-榄香烯诱导的细胞凋亡是通过线粒体途径的,同时激活caspase依赖性凋亡及caspase非依赖性凋亡2条凋亡途径.

OBJECTIVE To study the effects and its possible mechanism of β-elemene on apoptosis of human osteosarcoma MG-63 cells. METHODS The different mass concentration of β-elemene treated the MG-63 cells in vitro, and the survival rate of MG-63 cells was measured by MTT, the apoptosis of MG-63 cells was detected by flow cytometry, and the expression of apoptosis-related protein was determined by immunofluorescence and Western Blot. RESULTS As compared with the control group, the survival rate of MG-63 ceils treated with the β-elemene decreased significantly（P〈0.05）, and the expression of apoptosisrelated protein increased significantly（P%0.01）. CONCLUSION The β-elemene could induce apoptosis of human osteosarcoma MG-63 cells via a mitochondrial mediated pathway with the activation of both caspase-dependent and caspase-independent apoptotic pathways.