局部注射酸敏感离子通道抑制剂阿米洛利对胫骨癌痛大鼠疼痛行为学的影响

Pain relief effect of amiloride regional injection in rat model of tibial bone cancer

目的观察局部注射酸敏感离子通道抑制剂阿米洛利对胫骨癌痛的缓解作用.方法选取32只雌性 SD 大鼠,按随机数字表法分为4组,空白对照组（Cont 组）、假手术组（Sham 组）、骨癌痛组（BCP 组）和骨癌痛联合阿米洛利组（A 组）,各8只.BCP 组和 A 组大鼠在胫骨上端骨髓腔内注射5滋l Walker-256乳腺癌细胞制备胫骨癌痛模型,建模成功后第7天于胫骨上端区分别注射0.9%氯化钠溶液和阿米洛利溶液各100滋l;Sham 组大鼠胫骨上端骨髓腔内注射等体积 Hank 液,并于相同时间点在胫骨上端区注射0.9%氯化钠溶液100滋l.分别于注射 Walker-256乳腺癌细胞或 Hank 液前1天（T0）、注射后第7天（T1）、注射阿米洛利或0.9%氯化钠溶液后30min（T2）、1 h（T3）、2 h（T4）、4 h（T5）、8 h（T6）时检测各组大鼠机械缩足阈值（MWT）和自由行走疼痛评分.结果与T0比较,BCP 组和 A 组各时点 MWT 值、自由行走疼痛评分的差异有统计学意义（P〈0.05）;与 Cont 组相比,BCP 组和 A 组各时点MWT 值、自由行走疼痛评分的差异有统计学意义（P〈0.05）;与 BCP 组比较,A 组 T2~T5MWT 值、自由行走疼痛评分的差异有统计学意义（P〈0.05）;与 T1时比较,A 组 T2~T5 MWT 值、自由行走疼痛评分的差异有统计学意义（P〈0.05）.结论局部注射酸敏感离子通道抑制剂阿米洛利可有效缓解胫骨癌痛大鼠的疼痛行为.

Objective To assess the pain relief effect of amiloride regional injection in a rat model of tibial bone cancer pain. Methods Thirty-two female SD rats were randomly divided into 4 groups （n=8 in each group） ： Cont group, Sham group, bone cancer pain group（BCP group） , bone cancer pain and amiloride group（A group）. Fibial bone cancer pain was induced by injection of breast cancer Walker-256 cel s into marrow cavity of upper tibia bone in BCP and A groups, amiloride （A group） or normal saline（BCP group） were injected around upper tibia bone at d7 after the model induced. Rats in Sham group were injected with Hank solution into marrow cavity of upper tibia bone, and normal saline around upper tibia bone at the same time points. The mechanical withdrawal threshold （MWT） was measured at d1 before（T0） and d7 after injection（T1） of Walker-256 cel s or Hank＇s solution; 30 min（T2） and 1（T3）, 2（T4）,4（T5） and 8h（T6） after injection of amiloride or normal saline. Results Compared to the base-line, MWT was significantly decreased and the score of walk-associated ambulatory pain was increased in BCP and A group at all time points; compared to control group, MWT was significantly decreased and the score of walk-associated ambulatory pain was increased in BCP and A group at al time points; compared to BCP group, MWT was significantly increased and the score of walk-associated ambulatory pain was decreased at time points T2~T5 in A group; compared with T1, MWT was increased and the score of walk-associated ambulatory pain was decreased in A group at time points T2~T 5. Conclusion Regional injection of amiloride can reduce mechanical al odynia in a rat model of tibial bone cancer pain.