百草枯和氧桥氯甲桥萘对离体大鼠中脑多巴胺神经元的损害

Damage of Paraquat and Dieldrin on Cultured Rat Dopaminergic Neurons

目的 建立大鼠中脑多巴胺神经元的培养体系 ,判断百草枯 (Paraquat)和氧桥氯甲桥萘 (Dieldrin)能否对培养的大鼠中脑多巴胺神经元产生选择性损害。方法 将 0 .0 0 1～ 1 0 0 μmol/L浓度的Paraquat、Dieldrin加入到原代培养的大鼠中脑多巴胺神经元中 ,计数存活的多巴胺神经元和非多巴胺神经元。结果 Paraquat对大鼠中脑多巴胺神经元无选择性损害。 1 μmol/LDieldrin能选择性损害大鼠中脑多巴胺神经元。 结论 Dieldrin可能是致帕金森病 (PD)的神经毒物 。

Purpose To establish the culture system of rat dopaminergic neurons,and to determine whether Paraquat and Dieldrin are selectively toxic for cultured rat dopaminergic neurons respectively. Methods The cultured rat dopaminergic neurons were treated for 24 h with Paraquat and Dieldrin (0.001 to 100 μmol/L)respectively.Data were expressed as percentage of surviving TH(tyrosine hydroxylase) positive(TH +) cells and non TH positive (TH +) cells per culture dish. Results Paraquat was not selectively toxic for TH + neurons.Dieldrin (1 μmol/L) selectively decreased the number of TH + neurons without affecting non TH positive (TH +) cells.The EC 50 of Dieldrin on TH + neurons was 27.6 μmol/L. Conclusions Paraquat can not selectively destroy dopaminergic neurons in culture.Dieldrin (1 μmol/L) can selectively destroy the dopaminergic neurons in culture,which makes it a potential etiological agent for Parkinson's disease.The possible parkinsonogenic effect of Dieldrin is deserved for further investigation.