沙眼衣原体感染输卵管性不孕患者输卵管液中肿瘤坏死因子α和白细胞介素6的测定

Determination of Tumor Necrosis Factor alpha and Interleukin-6 Levels of the Tubal Fluids in Patients with Infertility Caused by Infection of Chlamydia Trachomatis

目的 探讨沙眼衣原体 (CT)感染所致输卵管性不孕患者输卵管液中肿瘤坏死因子α(TNF α)和白细胞介素 6 (IL 6 )的水平及其意义。方法 用酶联免疫吸附试验法测定 2 2例CT感染输卵管性不孕 (A组 )、2 3例非CT感染输卵管性不孕 (B组 )及 19例已生育输卵管正常妇女 (对照组 )输卵管液中TNF α和IL 6的水平。结果 A组TNF α的水平为 178ng/L ,高于对照组的 12 4ng/L(P<0 .0 1) ;A组中输卵管阻塞者TNF α为 199ng/L ,高于粘连者的 142ng/L(P <0 .0 1)。A组IL 6的水平为 6 81ng/L ,高于B组的 2 6 4ng/L及对照组的 2 2 9ng/L(P均 <0 .0 1) ,B组与对照组之间比较差异无显著性 (P >0 .0 5 )。结论 无症状输卵管CT感染可使输卵管液中TNF α和IL 6升高 ,其中TNF α与输卵管损伤程度有关。TNF α越高 。

Objective To study the levels of tumor necrosis factor alpha (TNF α) and interleukin 6 (IL 6) in tubal fluids of tubal factor infertile (TFI) women due to chlamydia trachomatis (CT) infection and its implication. Methods Twenty two TFI women with CT infection (group A), twenty three TFI women without CT infection (group B) and nineteen fertile women with normal tubes (group control) were enrolled for study. Levels of TNF α and IL 6 in tubal fluids were measured by enzyme linked immunosorbent assay. Results Group A had significantly higher TNF α levels than those of group control (178 ng/L versus 124 ng/L, P <0.01). Moreover, women with tubal blocks had significantly higher TNF α than those of tubal adhesions (199 ng/L versus 142 ng/L, P <0.01). IL 6 levels of group A were significantly higher than those of group B and group control (681 ng/L versus 264 ng/L and 229 ng/L, both P <0.01),but there is no difference between group B and group control. Conclusions Local TNF α and IL 6 levels increased in silent tubal infection of CT. TNF α had a positive relevance with the degree of tubal damage.