雌激素对去势兔血脂、内皮素及动脉粥样硬化形成的影响

Effects of Estrogen on Serum Lipids,Plasma Endothelin-1,Atherosclerosis in Ovariectomized Rabbits

目的 探讨雌激素对卵巢切除 (OVX)的去势兔的血脂、内皮素、动脉粥样硬化斑块形成的影响。方法 将 45只 3月龄雌兔随机分为 5组 ,每组 9只。A组为正常对照 ,B组为假手术 +胆固醇饮食 ,C组为OVX +胆固醇饮食 ,D组为OVX +胆固醇饮食 +苯甲酸雌二醇 ,E组为OVX +胆固醇饮食 +戊酸雌二醇。喂养 12周后取血 ,分别测定血脂、内皮素 ,处死动物并留取主动脉标本测量动脉粥样硬化斑块的面积。结果 (1)D组和E组总胆固醇 (TC)分别为 (2 4.95± 5 .31)mmol/L和 (2 7.5 3±13.18)mmol/L ,与C组 (37.6 9± 4.16 )mmol/L相比较 ,差异有显著性 (P <0 .0 5 ) ,与B组相比较 ,差异无显著性。用药各组高密度脂蛋白胆固醇 (HDL C)比较 ,差异无显著性。C组低密度脂蛋白胆固醇(LDL C)为 (4.0 5± 1.34 )mmol/L ,E组为 (2 .6 7± 1.0 2 )mmol/L ,两者相比较 ,差异有显著性 (P <0 .0 1)。(2 )D、E组血浆内皮素 1(ET 1)分别为 (2 6 2 .19± 36 .45 )ng/L和 (2 45 .37± 35 .76 )ng/L与C组的(34 3.37± 10 6 .10 )ng/L相比较 ,差异有显著性 (P <0 .0 5 )。 (3)D、E组动脉粥样硬化斑块面积占主动脉总面积的百分比分别为 (17.30± 5 .44 ) %和 (36 .99± 2 0 .2 9) % ,显著低于C组的 (74.83± 12 .5 8) %(P <0 .0 5 ) ,与B组的

Objective To investigate the effects of estrogen on serum lipids, plasma endothelin 1(ET 1) and atherosclerosis in ovariectomized(OVX) rabbits. Methods Forty five female rabbits were randomly divided into 5 groups, there are 9 in each: group A: normal control; group B: sham operation + cholesterol feeding; group C: OVX + cholesterol feeding; group D: OVX + cholesterol feeding + estradiol benzoate; group E: OVX + cholesterol feeding + estradiol valerate. The OVX rabbits fed with cholesterol were used as a model for atherosclerosis. After fed for 12 weeks, all rabbits were sacrificed, and the levels of serum lipids, plasma ET 1, atherosclerosis plaque of aorta were measured. Results (1) Simillar with group B, the total cholesterol (TC) of group D and E [(24.95±5.31)mmol/L and (27.53±13.18)mmol/L respectively], were significantly decreased as compared with group C which was (37.69±4.16)mmol/L. (2) The plasma ET 1 level of group C, which was (343.37±106.10) ng/L, was significantly higher as compared with group D and E, which were (262.19± 36.45) ng/L和(245.37±35.76) ng/L respectively. (3) There were no statistically differences of lipidic sedimentation area among group D,E,and B, which were (17.30±5.44)% and (36.99±20.29)% and (29.03± 9.67)% respectively. All of them were significantly decreased as compared with group C, which was (74.83±12.58)%. Conclusion Estrogen may improve the serum lipids, decrease the plasma ET 1 level, and prevent the atherosclerosis of ovariectomized rabbits.