姜黄素对环磷酰胺所致的小鼠睾丸组织氧化损伤的保护作用

The effects of curcumin on mouse testis oxidative damage induced by cyclophosphamide

目的探讨姜黄素（Cur）对抗环磷酰胺致小鼠睾丸组织氧化损伤的作用.方法成年昆明小鼠随机分成4组：正常组、模型组,低、高剂量Cur组.模型组和Cur低、高剂量Cur组小鼠按照40mg/kg,腹腔注射环磷酰胺（CTX）,连续5d,同时低、高剂量Cur组小鼠每天给予Cur混悬液100、200 mg/kg灌胃;正常组及模型组小鼠予等量0.5%羧甲基纤维素钠灌胃.共28d.末次给药24 h后,处死全部小鼠,观察小鼠附睾精子活动率、畸形率、低渗膨胀率,睾丸组织匀浆后测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和活性氧（ROS）含量.结果与模型组相比,Cur组精子活动率、低渗膨胀率、睾丸组织SOD与GSH-Px 含量等均明显升高（P〈0.05、P〈0.01）,精子畸形率和MDA、ROS含量有明显降低（P〈0.05、P〈0.01）.结论适量的Cur可对抗环磷酰胺所致小鼠睾丸组织的损害,其作用机制可能与Cur清除氧自由基,抗氧化作用有关.

Objective To explore the effect of curcumin against cyclophosphamide-induced mouse testis oxidative damage. Methods Divide adult Kun Ming mice into four groups randomly. Those are normal group, model group, low curcumin treatment group, high curcumin treatment group. As for the model group, low curcumin treatment group and high curcumin treatment group, imply 40mg/kg intraperitoneal injecton of cyclophosphamide （CTX）for five days. Normal group and model group have the same amount of 0.5%sodium carboxymethyl cellulose gavage. The process contains 28 days. 24 hours after the last injection, kill all the mousse. To observe mouse epididymal sperm motility rate, malformation rate, hypotonic swelling, after mouse testis homogenate determinate superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and malondialdehyde （MDA）, reactive oxygen species （ROS） content. Results Compare with the model group, in curcumin group, sperm motility, hypotonic swelling, testicular tissue SOD and GSH- Px content, etc increase obviously （P〈0. 05, P〈0.01）, sperm deformity rate, MDA, ROS content decrease obviously（P〈0.05, P〈0.01）. Acridine orange staining（AOT）show that compare with model group, the mouse sperm DNA integrity of curcumin group increase obviously which is statistically significant （ P〈0.01）. Conclusion The right amount of curcumin can against cyclophosphamide-induced mouse testis damage. Its mechanism may be related to the scavenge oxygen free radicals and antioxidant effect of curcumin.