α-谷胱甘肽-S-转移酶监测肝移植术后肝脏功能的研究

The value of serum α glutathione-S- transferase monitoring the hepatic function following liver transplantation

目的 探讨肝移植术后监测α 谷胱甘肽 S 转移酶 (α GST)的价值。方法 建立大鼠原位肝移植模型 ,并将模型分为同种异体移植组、同基因移植组和单纯开腹 3组 ,动态监测血清α GST、谷丙转氨酶 (ALT)、谷草转氨酶 (AST)活性变化。结果 非排斥组α GST在术后 2d内恢复正常 ,ALT、AST需要 3d后才恢复正常。排斥组α GST每天平均变化率与ALT、AST相比差异有显著性(P <0 .0 5 ) ,自术后第 3天起A、B组间α GST水平差异有显著性 (P <0 .0 5 )。结论 血清α GST可作为反映肝脏急性排斥时肝细胞损伤的有效指标 ,其敏感性明显高于其他肝功能指标 ,但特异性与ALT、AST差异无显著性。

Objective\ To evaluate the value of serum α-GST monitoring the hepatic function following liver transplantation. Methods\ The rat orthotopic liver transplantation model was established. The rats were divided into allogeneic group, syngeneic group and control group. The activities of α-GST, ALT and AST following liver transplantation dynamically determined. Results\ In syngeneic group, α-GST levels normalised within 48 h, but ALT and AST levels returned to normal on day 3 or day 4. In allogeneic group, there was significantly higher rate of median change per day in α-GST than that in ALT and AST. There were statistical differencnes in α-GST levels from the day 3 between allogeneic and syngeneic groups. Conclusions\ Serum α-GST as an sensitive marker of hepatocellular damage was associated with acute liver rejection. α-GST was a more sensitive but probably less-specific marker of the hepatocellular integrity than conventional liver function tests.\;