肿瘤坏死因子-α对肝星状细胞基质分解素-1及其抑制因子基因表达的调节

Regulation of stromelysin-1 and TIMP-1 gene expression in hepatic stellate cells by tumor necrosis fact or-α

目的 探讨肿瘤坏死因子 α(TNF α)对肝星状细胞基质分解素 1及金属蛋白酶组织抑制因子 1(TIMP1)基因表达的影响。方法 在培养的肝星状细胞系中加入 30 μg/LTNF α,于 8、2 4、48、72h 4个不同的时间点收集细胞 ,提取总RNA ;用逆转录定量聚合酶链反应 (PCR)方法测定基质分解素 1及TIMP1的基因表达水平。结果 TNF α组肝星状细胞基质分解素 1基因表达水平在 8、2 4、48、72h 4个时间点均明显高于对照组 ;2 4～ 48h达高峰 ,为对照组的 2倍。TNF α组肝星状细胞TIMP1的基因表达水平在 8、2 4、48h与对照组差异无显著性 (P >0 .0 5 ) ,72h显著升高 ,为对照组的近 3倍。结论 TNF α可增强肝星状细胞基质分解素 1及TIMP1基因的表达。

Objective\ To investigate the effects of tumor necrosis factor-α (TNF-α) on stromelysin-1 and tissue inhibitor of metalloproteinase-1 (TIMP-1) gene expression in hepatic stellate cells. Methods\ The hepatic stellate cell line (HSC-T6) was cultured in medium containing TNF-α (30 μg/L) and the cells were collected at different incubation time points (8, 24, 48 and 72 h). Total RNA of HSC was extracted. The gene expression levles of stromelysin-1 and TIMP-1 were detected by using reverse transcription polymerase chain reaction method. Results\ Stromelysin-1 gene expression level in TNF-α group was obviously higher than those in control group at the time points of 8, 24, 48 and 72 h, peaked at 24 to 48 h (2 to 3 times higher than in control groups)1. No significant difference in TIMP-1 gene expression level was found between the two groups at 8 to 48 h. However, TIMP-1 gene expression level in TNF-α group was increased remarkably at 72 h and was 3 times higher than those in the control group. Conclusion\ TNF-α could promote gene expression of stromelysin-1 and TIMP-1 in hepatic stellate cells.\;