缺血再灌注对大鼠肺细胞间粘附分子-1表达的影响

Effect of ischemia/reperfusion on the expression of intercellular adhesion molecule-1 (ICAM-1) in rat lung

目的 观察缺血再灌注 (I/R)对肺组织细胞间粘附分子 1(ICAM 1)表达的影响 ,分析ICAM 1表达与肺内中性粒细胞浸润的关系。方法 Wistar大鼠单肺原位热缺血再灌注损伤模型分7组 ,左肺缺血 90min ,再灌注时间分别为 0、1、2、4、8、16、2 4h。逆转录 聚合酶链式反应 (RT PCR)法及Westernblot法检测肺组织ICAM 1mRNA及蛋白表达 ,测定各组肺组织髓过氧化物酶活性。结果 与再灌注开始时比 ,再灌注 1hICAM 1mRNA升高 1.5倍 ,16h降为正常 ;再灌注 4hICAM 1蛋白开始升高 ,8h升高 1倍 ,此后逐渐下降。再灌注 8～ 2 4h髓过氧化物酶活性与ICAM 1蛋白表达正相关 (γ =0 .6 9,P<0 .0 1)。结论 I/R可引起大鼠肺ICAM 1升高表达 ,再灌注 8～ 2 4h肺组织中性粒细胞浸润与ICAM 1表达呈正相关。

Objective\ To evaluate the effect of ischemia/reperfusion (I/R) on the expression of intercellular adhesion molecule-1 (ICAM-1) in lung and study the relationship between expression of ICAM-1 and polymorphonuclear leukocyte (PMN) infiltration.Methods\ Single lung in suit warm I/R animal model was used. The rats were subjected to 90 min left lung ischemia followed by 0,1,2,4,8,16 or 24 h of reperfusion separately. At the end of reperfusion, expression of ICAM-1 mRNA and protein was detected by using semi-quantitative RT-PCR and Western blot method. Myeloperoxidase activity in each group was also measured. Results\ ICAM-1 mRNA expression was increased at 1 h of reperfusion, and decreased to basal level 16 h after reperfusion. Expression of ICAM-1 protein began to increase 4 h after reperfusion, peaked at 8 h of reperfusion and then gradually decreased. Myeloperoxidase activity was positively correlated with ICAM-1 expression during 8 to 24 h of reperfusion (r=0.69, P<0.01).Conclusion\ I/R could upregulated ICAM-1 expression in rat lung, and the PMN infiltration after 8 h of reperfusion was correlated with the expression of ICAM-1 in lung. \;