磁性药物载体脑定向分布对肝肾及骨髓的影响

Effects of targeted brain distribution of FM-HSA-MTX carrier on liver, kidney and bone marrow

目的 研究磁导向下磁性药物载体脑定向分布 ,磁性药物载体对肝、肾及骨髓的毒副作用 ,为脑胶质瘤的磁导向化疗提供可靠的依据。方法 昆明小鼠 5 0只 ,将一定量的磁性药物载体混悬液通过尾静脉注入小鼠体内 ,头部一侧放置聚焦磁场 ,定期给药观察 3个月 ,定时取血检查红细胞、白细胞、血小板、谷丙转氨酶 (ALT)、非蛋白氮 (NPN) ,并设立对照组。结果 7、14d磁导向组红细胞为(935 .0± 79.2 )、(92 5 .0± 81.5 )万 /mm3 ,肝功能及肾功能的变化不大 ,非磁导向组 (MTX组 )红细胞为(5 70 .0± 82 .2 )、(5 0 5 .0± 81.2 )万 /mm3 ,肝功能及肾功能的变化较大 ,两者差异有非常显著性(P <0 .0 1)。结论 磁导向下磁性药物载体定向分布于大脑靶区 ,改变了MTX随血流分布的特性 ,MTX在大脑定向浓集 ,而在肝、肾及骨髓的蓄积明显减少 ,显著减轻了对肝、肾及骨髓的损害作用。

Objective To study the targeted brain distribution of FM-HSA-MTX carriers under magnetic guidance and side-effects of the carriers on liver, kidney and bone marrow. To provide reliable evidence for magnetic targeting chemotherapy for malignant glioma.Methods Fifty Kunming mice were used. FM-HSA-MTX carriers were injected into the mice regularly via tail vein. Magnetic field was placed near the right brain of mice. The mice were observed for 3 months. The mice blood was taken out regularly to determine RBC, WBC, BPC, SGPT and NPN respectively. Results At 7 d and 14 d, RBC (935.0±79.2), (925.0±81.5),ALT, NPN had no obvious change in magnetic targeting group (FM-HSA-MTX group), while RBC (570.0±82.2), (505.0±81.2), SGPT and NPN changed evidently in MTX group (P<0.01). Conclusions Under magnet guidance, FM-HSA-MTX carriers was distributed to brain targeting area, which may change the characteristics of MTX flow distribution. The collection of MTX was reduced in liver, kidney and bone marrow, alleviating the damage to them.