蒙药瞿麦中多糖超声提取工艺研究及含量测定

Extraction optimization and content determination of polysaccharide in Mongolian medicine Dianthus superbus L.

目的研究瞿麦中多糖的超声提取方法及其含量测定的方法。方法采用苯酚-硫酸法显色,并以紫外-可见分光光度计测定瞿麦中多糖含量,以其作为评价指标,先后通过单因素试验和正交试验来确定提取瞿麦多糖的最佳工艺条件。结果超声法提取瞿麦中多糖的最佳工艺为料液比为1:20,温度60℃,超声时间为30 min,提取2次。各因素影响顺序为提取温度>提取时间>提取次数>料液比,测得瞿麦中多糖含量为5.463%,平均回收率为98.6%,RSD=1.5%(n=6)。结论以上工艺为运用超声技术从瞿麦中提取出多糖的最佳工艺。采用紫外可见分光光度法测定瞿麦中多糖含量的方法简便、快速、灵敏,结果准确可靠,可作瞿麦中多糖的含量测定方法。

Objective To explore the extraction and determination of polysaccharide in the Mongolian medicine Dianthus superbus L.. Methods Phenol-sulfuric acid method and UV-PDA spectrophotometer were used to determine polysaccha- ride, which was used as the evaluation index to determine the optimum technological conditions ofpolysaccharide extraction in Dianthus superbus L. by a single factor and orthogonal experiments. Results The best ultrasonic extraction of polysac- charide in Dianthus superbus L. was as follows： solid-liquid ratio at 1 ： 20, 60 ℃, ultrasonic extraction 30 rain, extracting twice. The order of the 4 factors was extraction temperature〉 extraction time 〉 number of extraction 〉 solid-liquid ratio. The polysaccharide content measured in Dianthus superbus L. was 5.463%; the average recovery rate was 98.6%, RSD = 1.5% （n = 6）. Conclusion The above extraction is the best ultrasonic extraction ofpolysaccharide in Dianthus superbus L.. UV-Vis spectrophotometric method is simple, rapid, sensitive, accurate and reliable, which can be used to determine poly- saccharide in Mongolian medicine Dianthus superbus L..