实时荧光定量PCR方法检测食管癌中PLCE1基因的异常表达

Real-time fluorescent quantitative PCR assay for detection of ESCC tissue the PLCE1 gene expression

目的:检测新疆哈萨克族食管癌和癌旁组织中PLCE1基因表达水平,分析PLCE1基因表达与食管鳞癌的分化、分期及转移的相关性。方法:选取30例食管鳞癌及30例配对癌旁组织,应用实时荧光定量PCR(SYBRGreen Real-time PCR)检测PLCE1基因的mRNA表达,分析其与食管鳞癌临床病理特征的关系。结果:PLCE1mRNA在食管鳞癌的表达水平明显高于在癌旁正常组织的表达,差异具有统计学意义(P=0.012);PLCE1mRNA的表达与肿瘤分期、淋巴结转移相关,差异具有统计学意义(P<0.05);与肿瘤部位、肿瘤大小及分化程度无关,差异无统计学意义(P>0.05)。结论:PLCE1与食管鳞癌的分期、淋巴转移有关,而与食管鳞癌的分化程度无明显关系。提示PLCE1的高表达在食管鳞癌的发生、浸润及转移过程中可能具有重要作用。

Objective： To investigate the expression of PLCEI gene mRNA in Kazakhg esophagas squamous cell carci- noma （ESCC） and explore the relationship of PLCE1 expression to the differentiation degree ,stage ,invasion and metastasis of ESCC. Methods：real-time fluorescent quantitative PCR assay was employed to detect the expression of PLCE1 mRNA in 30 paraffin-embedded Xinjiang Kazakh ESCC and 30 non-cancerous adjacent tissue（NCAT）. Restflts：The expression of PLCEI mRNA in ESCC was significant higher than that in normal-appearing mueosa（P =0. 022）;the expression of PLCE1 mRNA was significantly associated with regional lymph node metastasis （ P = 0. 002 ） and pathological staging（ P = 0.022）, the expres- sion of PLCE1 mRNA had no relationship with the degree of differentiationgender ,age,tumor location and tumor size （P 〉 0. 05）. Conclusion：The over-expression of PLCEI mRNA was related to the genesis or development or metastasis of ESCC and might be a useful parameter as an indication of tumor invasion, metastasis, as well as a noval target site for gene therpy.