砷对小鼠成神经瘤细胞N2a的毒性作用

NaAsO2 Induced Neurotoxicity in Mouse Neuroblastoma Cells N2a

目的:观察亚砷酸钠对小鼠成神经瘤细胞系N2a细胞的毒性作用。方法:将不同浓度亚砷酸钠对小鼠成神经瘤细胞N2a进行染毒,采用细胞毒性试验(MTS)法观察NaAsO2对N2a细胞的毒性作用,Hoechst33258荧光染色观察凋亡细胞的形态,采用AnnexinV-FITC/PI双重染色的流式细胞检测细胞凋亡率,Caspase-3试剂盒检测Caspase-3酶活性。结果:不同浓度的NaAsO2(10,20,30,40和50μmol/L)作用于N2a细胞,24h后均引起了细胞明显的形态学变化,促进细胞凋亡率,且浓度越大,凋亡效果越明显。结论:NaAsO2对N2a细胞能产生神经毒性,导致细胞凋亡。

Objective： To investigate the toxic effects and possible mechanisms of NaAsO2 in the mouse neuroblasto- ma cell line （N2a）. Methods：Cultured N2a cells were exposed to NaAsO2（ 10,20,30,40 and 50p, mol/L） for 24h. MTS assay was used to evaluate the cell viability. A flow cytometry assessment with Armexin V-FITC/PI and apoptotic morpholo- gy were used to detect apoptosis induced by NaAsO2. Results：The results showed that NaAsO2 at 24 h substantially altered the morphology and significantly induced apoptosis （ P 〈 0.05 ）. Conclusion： An increased concentration of NaAsO2 elevat- ed the apoptotic effect in N2a cells.