Beclin-1蛋白在霍奇金淋巴瘤中的表达及其临床意义

Expression and Clinical Significance of Beclin-1 in Hodgkin Lymphoma

目的:探讨beclin-1蛋白在霍奇金淋巴瘤(Hodgkin lymphoma,HL)组织霍奇金里斯细胞(Hodgkin Reed-Sternberg,HRS)的表达及其与HL患者临床特征之间的关系。方法:收集2001年1月至2012年1月青岛大学医学院附属医院初发的HL患者61例和同期反应增生性淋巴结炎患者35例的组织标本为研究对象,通过免疫组化检测beclin-1蛋白在HL组织HRS细胞、反应性细胞及反应增生性淋巴结组织中的表达情况。结果:①HL与反应增生性淋巴结组织中beclin-1蛋白的阳性表达率分别为64%(39/61)和86%(30/35),HL组的beclin-1蛋白表达明显低于反应增生性淋巴结炎组,两者之间差异有统计学意义(P<0.05);而在同一HL组织中,HRS细胞beclin-1蛋白的表达明显低于反应性细胞,差异有统计学意义(P<0.05)。②不同组织分型的HRS细胞beclin-1蛋白的表达有差异,淋巴细胞消减型(lymphocyte depletion,LD)HRS细胞beclin-1蛋白的表达明显低于混合细胞型(mixedcellularity,MC)及结节硬化型(nodular sclerosis,NS),差异有统计学意义(P<0.05),富于淋巴细胞型(lymphocyte rich,LR)与混合细胞型、富于淋巴细胞型与结节硬化型、富于淋巴细胞型与淋巴细胞消减型、混合细胞型与结节硬化型之间HRS细胞beclin-1蛋白表达差异无统计学意义(P>0.05)。③HL组织中HRS细胞beclin-1蛋白表达水平与患者Ann Arbor分期、B症状及血清LDH值有关(P<0.05),而与年龄、性别、巨大肿块及血沉无关(P>0.05)。结论:Beclin-1蛋白在HL中HRS细胞的表达明显降低,且与HL的恶性程度及侵袭性明显相关;beclin-1表达降低可能参与了HL发生发展。

Objective： To explore the expression of beclin-1 in Hodgkin lymphoma and the relationship between the expression of beclin-1 and the clinical characteristics.Methods： Immunohistochemical method was used to detect the expression of beclin-1 in 61 cases of Hodgkin lymphoma and 35 cases of reactive hyperplasia lymph node tissues who were admitted in our hospital from January 2001 to January 2012.Results： ①The positive expression rate of beclin-1 in Hodgkin lymphoma and the reactive hyperplasia lymph node were 64 %（39/61） and 86 %（30/35）,respectively,which was significantly lower in HL than that in the reactive hyperplasia lymph node（P0.05）;in the same HL tissue,the expression of beclin-1 in HRS cells was significantly lower than that in the reactive cells（P0.05）.②The expression of beclin-1 in HRS cells of LD was lower than that of MC and NS（P0.05）,but there was no significant difference of beclin-1 expression between in HRS cells of LR and MC,LR and NS,LR and LD,MC and NS（P0.05）.③There was significant correlation between beclin-1 expression and Ann Arbor stage,B symptoms and serum LDH（P0.05）,but there was no significant correlation between beclin-1 expression and age,sex,bulky disease and ESR（P0.05）.Conclusion： The expression of beclin-1 in HRS cells of Hodgkin lymphoma was down-regulated,the decreased expression of beclin-1 was related to the tumor malignancy and invasion,which may be involved in the tumorigenesis and progression of HL.