张家口地区献血者隐匿性乙肝病毒感染研究

Study of OBI among Blood Donors in Zhangjiakou

目的:对张家口地区无偿献血者中隐匿性乙肝病毒感染情况进行流行病学调查,为当地血液安全筛查提供指导意义。方法:应用HBsAg ELISA检测试剂盒对回库无偿献血者标本进行检测;对血浆标本采用nested-PCR技术进行HBV核酸检测;对阳性标本进行HBV DNA序列分析。结果:在总计5498例次标本的检测中,共有5417例为HBsAg阴性;HBsAg阴性标本中nested-PCR方法检出13例HBV DNA阳性(0.24%,13/5417);测序结果显示隐匿性HBV感染者中C基因型所占的比例为61.5%(10/16),明显高于HBsAg阳性的HBV感染者(28.2%,11/39,P<0.01)。结论:张家口地区无偿献血者中存在较高比例的隐匿性乙肝病毒感染。

Objective： To study occult hepatitis B virus infection（OBI） in blood donors in Zhangjiakou.Methods： HBsAg ELISA and Nested-PCR were used to detect HBV infection in the plasma samples of blood donors.NAT-reactive samples were subjected to HBV DNA sequence analysis.Results： A total of 5498 samples were assayed,in which 81 were HBsAg positive and 5417 HBsAg negative.In HBsAg negative samples,13 were tested HBV DNA positive by nested-PVR.The results of HBV DNA sequence analysis indicated that genotype C had a higher proportion in OBI cases than in HBsAg positive cases（61.5% vs 28.2%,P0.01）.Conclusion： There is a high proportion of occult hepatitis B virus infection in blood donors in Zhangjiakou.