二烯丙基二硫作用于K562细胞后凋亡相关基因的差异表达

Effects of Diallyl Disulfide on Differential Expression of Apoptosis Associated Genes in Leukemia Cell Line K562

目的:研究二烯丙基二硫(diallyl disulfide DADS)作用于人白血病K562细胞后凋亡相关基因的差异表达,为进一步探讨DADS诱导K562细胞凋亡的分子机制提供基础。方法:采用瑞士-吉姆萨和Hoechst 33342染色观察细胞形态学变化。运用基因芯片技术检测40 mg/LDADS作用于K562细胞24h后凋亡相关基因的差异表达,选择其中上调基因GADD45A、下调基因HO-1运用RT-PCR技术进行验证。结果:40 mg/L的DADS作用于K562细胞后出现凋亡所具有的典型形态学变化。40 mg/L DADS作用K562细胞24 h后有14个凋亡相关差异表达基因。GADD45A、HO-1基因表达情况与基因芯片结果一致。结论:DADS可能通过多个基因和多条信号转导通路共同作用诱导人白血病细胞K562凋亡。

Objective： To study the differentially expressed genes of apoptotic in K562 cells induced by Diallyl disulfide（DADS）.Methods： The morphologic changes of leukemia K562 cells after DADS treated were observed by Wright-Gimsa staining and Hoechst 33342 staining.The expression profile of apoptosis-associated genes in K562 cells,with or without 24-hour treatment of DADS（40mg/L）,was identified by gene array.The up-regulated GADD45A gene and down-regulated HO-1 gene were confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Results： DADS can induced the apoptosis of K562 cells.When treated with 40 mg/L DADS for 24 h,the differential expression of 14 apoptosis-associated genes were found by using gene array.The expression of GADD45A,HO-1 detected by RT-PCR were consistent with those detected by gene array.Conclusion： DADS induces apoptosis in K562 cells,which might be mediated by some specific genes and various singnal transduction pathways.