Oct4在晚期浆液性卵巢癌中的表达及意义

Expression of Oct4 Protein in Late-stage Serous Ovarian Cancer

目的:卵巢癌是女性生殖器官常见的恶性肿瘤,肿瘤干细胞理论认为卵巢癌中存在肿瘤干细胞,并且肿瘤干细胞是肿瘤复发、转移、耐药的罪魁祸首。Oct4是细胞干细胞特性的重要标志之一。本文旨在探讨Oct4蛋白在晚期浆液性卵巢癌的表达情况,并分析其表达水平与患者临床病理特征之间的关系。方法:采用免疫组化法检测101例晚期浆液性卵巢癌中Oct4蛋白的表达情况,并结合临床资料分析Oct4蛋白的表达水平在晚期浆液性卵巢癌患者中的意义。结果:晚期浆液性卵巢癌患者中Oct4阳性表达率为87.1%(88/101),其表达水平和患者的年龄、病理分化程度、淋巴结转移情况及临床分期无明显相关性(P>0.05),但是其高表达与化疗耐药明显相关(P=0.023)。结论:我们的研究证明Oct4在晚期浆液性卵巢癌中有较高表达,并且Oct4的表达与卵巢癌化疗后复发有关;理论上支持在晚期浆液性卵巢癌中可能存在肿瘤干细胞,且比例较高,肿瘤干细胞的比率和卵巢癌化疗耐药相关。Oct4可能是晚期浆液性卵巢癌治疗的新靶点。

Objective： Ovarian cancer is a common malignant tumors of the female genital system,cancer stem cell theory holds that the existence of cancer stem cells in ovarian cancer,and cancer stem cells is the culprit of tumor recurrence,metastasis and chemotherapy resistance.Oct4 is one of the hallmarks of cell stemness.Our research aims to investigate the expression of Oct4 protein in late-stage serous ovarian cancer by immunohistochemistry（IHC） and analyze its correlation with the clinical pathological features.Methods： The Oct4 protein expression was assessed by immunohistochemistry（IHC） in 101 specimens of late-stage serous ovarian cancer.A correlation was analyzed between Oct4 protein expression and clinical pathological features.Results： The positive rate of Oct4 rate was 87.1%（88/101）.Oct4 protein expression level was not related with age,lymph nodes metastasis,clinical stage and pathological differentiation（P 0.05）,while high Oct4 expression was associated with chemotherapy resistance（P = 0.023）.Conclusion： Our data represent firstly that Oct4 protein expression in late-stage serous ovarian cancer.Oct4 expression may contribute to chemotherapy resistance of serous ovarian carcinoma.Oct4 may be a promising therapy target for late-stage serous ovarian cancer.