一种利用花粉管结合农杆菌介导方法将iaaM基因导入玉米自交系的研究

Research on Transferring iaaM Gene to Maize Inbred Lines by Pollen Tube Pathway and Agrobacterium-mediated Method

[目的]利用花粉管结合农杆菌介导方法将iaaM基因导入玉米自交系。[方法]利用电击转化法将iaaM基因的表达载体转入农杆菌LBA4404菌株中,通过花粉管通道法将验证正确的菌株直接转化京24玉米自交系,最后通过除草剂筛选及PCR检测得到阳性植株。[结果]试验共得到12株抗性植株,其中10株为阳性植株。[结论]该研究成功建立了一种无需组织培养体系,直接利用花粉管通道进行农杆菌介导的玉米转化方法。

[Objective] The paper was to transfer iaaM gene to maize inbred lines by pollen tube pathway and Agrobacterium-mediated method.[Method] iaaM gene expression vector was introduced into Agrobacterium tumefaciens strain LBA4404 using the electric transformation method,and the verified correct strain was directly transformed to the Jing 24 maize inbred lines by pollen-tube pathway method.Finally,the positive plants were obtained by herbicide selection and PCR detection.[Result] The test obtained 12 resistant plants,and 10 of them were positive plants.[Conclusion] The study successfully established a transformation method for maize by pollen-tube pathway and Agrobacterium-mediated method without tissue culture system.