超声辅助菟丝子黄酮和多糖的连续提取工艺研究

Ultrasound-aided Extraction of Flavonoids and Polysaccharides in Cuscuta chinessis

[目的]优化超声辅助提取菟丝子黄酮和多糖的连续提取工艺。[方法]以菟丝子黄酮和多糖为提取对象,利用超声辅助法连续提取菟丝子黄酮和多糖,并通过设计正交试验优化了菟丝子黄酮和多糖超声辅助法连续提取工艺。[结果]试验表明,菟丝子黄酮的最佳提取工艺条件为超声功率600 W,乙醇浓度80%,提取时间40 min,固液比1∶30 g/ml。菟丝子多糖进行超声辅助提取的最佳条件为在提取温度为60℃条件下,超声功率200 W,提取2次,提取时间75 min,料水比1∶35 g/ml。[结论]研究可为菟丝子黄酮和多糖的综合研究开发提供依据,促进菟丝子资源的综合利用。

[Objective] To optimize ultrasound assisted continuous extraction technique of flavonoids and polysaccharides from Cuscuta chinessis.[Method] With flavonoids and polysaccharides from C.chinessis as extraction object,ultrasound assisted method was adopted for continuously extracting flavonoids and polysaccharides from C.chinessis,the orthogonal experiment was adopted to optimize the extraction technique.[Result] The results showed that the optimized conditions of extraction for flavonoids were as follows: the power is 600 W,the concentration of ethanol is 80%,the time is 40 minutes and the ratio of material to water is 1∶30 g/ml.The optimized conditions of extraction for polysaccharides were as follows: the power is 200 W,the extracting number of times is twice,the time is 75 minutes and the ratio of material to water is 1∶35 g/ml at 60 ℃.[Conclusion] The study will provide basis for comprehensive development of flavonoids and polysaccharides from C.chinessis,promoting comprehensive utilization of C.chinessis resource.