磷浓度对厌氧生物除磷效果及其微生物群落结构

Study on the effects of different phosphorus concentrations on anaerobic phosphorus removal and analysis of microbial community in the anaerobic phosphorus sludge

通过厌氧培养基试验,考察了不同起始磷浓度对厌氧生物除磷的影响,并用16S rDNA分子生物学技术PCR-DGGE方法对稳定时期的各种泥样品中微生物群落结构进行跟踪研究.试验结果表明:随着磷浓度的升高,总磷的去除量增加,当总磷含量高于50 mg.L-1时,总磷去除量不随初始磷浓度的升高而升高.稳定培养状态下微生物多样性丰富,不同起始磷浓度污泥样品相似性不高,其中初始磷浓度为20 mg.L-1和60 mg.L-1的厌氧污泥样品相似性最低为67.4%,磷浓度条件可改善微生物群落结构.

Through anaerobic culture test, the effects of different phosphorus concentrations on anaerobic phos- phorus removal were studied. Microbial community structures in the different sludge were investigated by 16S Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis （PCR-DGGE） method when the anaerobic systems were operated steadily. The results showed that the phosphorus removal quantity increased with the con- centration of phosphorus increasing. However, when the concentration of phosphorus was more than 50 mg ~ L^-1, the phosphorus removal will not increase with the concentration increasing. The DGGE fingerprints were analyzed by the software Quantity One to obtain the information of the microbial species in the different sludge, which showed that there was a high diversity in the bacterial communities, but the community similarity of dif-ferent phosphorus concentrations was not high. The lowest similarity of anaerobic sludge was only 67.4% when the concentrations of phosphorus were 20 mg·L^-1 and 60 mg·L^-1. The concentration of phosphorus can im-prove the microbial community in the anaerobic phosphorus sludge.