HtrA1在骨关节炎早期软骨退变中的诊断价值

Experimental Study of HtrA1 in the Pathogenesis of Osteoarthritis

目的：探讨骨关节炎患者关节滑液中HtrAl的表达是否与软骨退变程度相关。方法：研究对象包括膝骨关节炎（KOA）患者40例，其中软骨退变Ⅱ、Ⅲ、Ⅳ级的病例数分别为15、11、14例，正常人28例，应用ELISA测定丝氨酸蛋白酶1（HtrAl）在关节滑液中的表达程度。结果：KOAII、m、1v组中关节滑液的HtrAl因子表达均明显高于正常人，差异有统计学意义（P〈0．01），而不同软骨退变程度患者的关节液HtrAl因子表达也不同。KOAII、Ⅲ、1V组的组间HtrAl因子表达含量比较差异无统计学意义（P〉0．05）。正常对照组关节液HtrAl因子表达含量为67．89～208．33pg／m]，以208．33pg／ml作为阴性，KOA患者不同程度软骨退变（KOAII、Ⅲ、IV组）各组检测的敏感度均超过85．7％。结论：HtrAl对早期软骨退变（KOAm级）诊断的敏感度在90％以上，特别是诊断KOA患者KOAII级软骨退变的敏感度最高，可达93％以上。说明HtrAl是诊断早期软骨退变（KOAII级）的良好指标。

Objective： To explore the relationship of HtrA1 expression of synovial fluid in osteoarthfitis and the degree of cartilage degeneration. Method： The object of study, including 40 cases of patients with knee osteoarthritis, including cartilage degeneration grade II , m, grade 1V number of cases were 15, 11, 14, normal 28 cases, the application of ELISA, HtrA1 in synovial fluid the expression level.Result： KOA II, 1I, group IV the synovial fluid HtrA1 factor expression were significantly higher than normal, there was statistically significant （ P〈0.01 ）, the different cartilage degeneration patients the synovial fluid HtrA1 factor expression were also different.KOA 1I, m, IV group group HtrA1 factor expression levels was not statistically significant （ P〉0.05 ） .Normal control group joint fluid HtrA1 factor expression content to 67.89-208.33 pg / ml, if 208.33 pg / ml as a negative, the sensitivity of KOA I] , Ill, IV group were more than 85.7%.Conclusion： The HtrA1 early cartilage degeneration （ KOA grade III ） diagnostic sensitivity of 90%, in particular the diagnosis of patients with KOA KOA class II cartilage degeneration highest sensitivity, more than 93%. The HtrA1 description is a good indicator for the early diagnosis of cartilage degeneration （ KOA grade II ） .