东莞市病毒性腹泻病原学分析

Analysis of viral diarrhea pathogen in Dongguan city

目的了解东莞市病毒性腹泻的病原学分布情况。方法收集2010年7月至2012年9月东莞市东华医院感染性腹泻患者的粪便样品,采用ELISA方法检测轮状病毒,采用Realtime RT-PCR方法检测诺如病毒,采用RT-PCR方法检测星状病毒,采用Realtime PCR方法检测腺病毒。结果检测粪便样品共224份,诺如病毒、轮状病毒、腺病毒、星状病毒检测阳性率分别为17.9%、11.6%、4.5%,0.4%,总检测阳性率为31.3%。对其中22份轮状病毒阳性样品进行血清分型,结果G血清型中,G1型10株,G2型1株,G3型8株,G9型3株。P血清型中,P6型1株,P8型12株,9株未能分型。40株诺如病毒全部为GⅡ型。1株星状病毒经测序分析为1型。结论东莞市腹泻病毒主要感染3岁以下的婴幼儿,诺如病毒和轮状病毒为主要病原体,轮状病毒的分布呈多样性,以G1型为主。

Objective To understand the distribution of the main pathogens of viral diarrhea in Dongguan city. Methods The feces samples were collected from infectious diarrhea patients in Donghua Hospital. Rotavirus was detected by ELISA, norovirus was detected by Real-time RT-PCR. Astrovirus was detected by RT-PCR. Adenovirus was detected by Real-time PCR. Results Totally 224 samples were detected and the positive rates of norovirus, rotavirus, adenovirus and astrovirus were 17.9%,11.6%, 4.5% and 0.4%.Among the 22 rotavirus positive samples, the serotype G1,G2, G3, G9, P6, P8 were 10,1,8, 3,1,12. Nine strains failed to serotyping. All of the 40 norovirus strains were G II genotype and one astrovirus strains was identified as serotype I. Conclusion The patients infected with diarrhea virus were chiefly infants under 3 years old, norovirus and rotavirus were the main pathogens and the distribution of rotavirus serotype was diverse with serotype G1 was predominant.