葡萄糖对大鼠骨髓间充质干细胞向成骨细胞分化的影响

Effects of glucose on the differentiation of rat BMSC into osteoblastic lineages

目的体外观察葡萄糖对间充质干细胞(BMSCs)向成骨细胞(OB)分化的影响,探讨其对骨代谢的作用机制。方法从大鼠长骨骨髓分离获取间充质干细胞,在不同糖浓度(5.6mmol/l、25mmol/l、50mmol/l)干预下向成骨细胞诱导分化培养21d;茜素红染色、光镜计数矿化率;realtime PCR测定OB的标记物碱性磷酸酶(ALP),骨钙素(BGP)及转录因子Runx2 mRNA表达,进行不同糖浓度组干预后的变化比较。结果显示随着糖浓度升高,矿化率显著降低,差异有统计学意义(P<0.05)。与5.6mmol/l组比较,50mmol/l组的ALP,BGP及Runx2 mRNA表达减少,分别为73%(P<0.05)、69%(P<0.05)及70%(P<0.05),25mmol/l组的ALP、BGP及Runx2 mRNA表达下降也具有显著性(P<0.05)。结论高糖抑制BMSCs向OB分化,可能为糖尿病性骨质疏松形成的重要机制之一。

Objective To investigate the effects of glucose on the differentiation of rat bone-marrow stromal cells（BMSCs）（mesenchymal stem cell）into osteoblastic lineages in the presence of an osteogenic medium.Methods The calcium tubercle formation would be examined by staining of mineralized nodules with Alizarin red S.And the activity of alkaline phosphatase（ALP）,osteocalcin and Runx2 between interblocks was examined by realtime PCR.Results Twenty-one days after induction the number of osteoblasts decreased in higher concentration glucose as detected by Alizarinred S,the mRNA expression of ALP,BGP and Runx2 decreased to73%（P0.05）,69%（P0.05）and 70%（P0.05）,in 50mmol/l glucose than in 5.6mmol/l glucose.The differentiation of osteoblastics decreased in a dose-dependent manner with pioglitazone than without pioglitazone in triconcentration of glucose.Conclusions High concentration glucose inhibited the differentiation of BMSCs into osteoblastics,this observation provide a potential mechanisms of diabetes-induced osteoporosis.